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Genes Dev

Title:		MAT alpha 1 can mediate gene activation by a-mating factor
Author(s):		Sengupta P; Cochran BH;
Address:		"MIT Center for Cancer Research and Department of Biology, Cambridge, Massachusetts 02139"
Journal Title:		Genes Dev
Year:		1991
Volume:		5
Issue:		10
Page Number:		1924 - 1934
DOI:		10.1101/gad.5.10.1924
ISSN/ISBN:		0890-9369 (Print) 0890-9369 (Linking)
Abstract:		"In the yeast Saccharomyces cerevisiae, expression of alpha-specific genes is governed by the MAT alpha 1 and MCM1 gene products. MAT alpha 1 and MCM1 bind cooperatively to PQ elements upstream of alpha-specific genes. The PQ element not only directs alpha-specific expression but can also direct gene induction in response to treatment with a-mating pheromone. We have used gene fusions to investigate whether induction conferred by the PQ box is mediated through either MAT alpha 1 or MCM1, or a combination of both. When MCM1 is fused to the DNA-binding domain of the bacterial repressor LexA, this fusion protein is capable of trans-activating a lacZ reporter gene driven by a LexA operator. However, the transcriptional activity of the MCM1-LexA fusion is not further enhanced by treatment of cells with a-factor. A MAT alpha 1-LexA fusion protein is also capable of trans-activation through a LexA operator. Moreover, the activity of the MAT alpha 1-LexA fusion protein can be further induced by treatment with a-factor. When progressive deletions are made from the amino terminus of MAT alpha 1 in the fusion protein, the basal level of trans-activation progressively decreases, but the inducibility of the fusion protein increases. MAT alpha 1-LexA fusion proteins, which have greater than or equal to 57 amino acids deleted from the amino terminus of MAT alpha 1 are not capable of trans-activation. In addition, the activity of the MAT alpha 1-LexA fusion protein is dependent on the functions of the STE7, STE11, and STE12 genes that encode components of the pheromone response pathway"
Keywords:		"Amino Acid Sequence Animals Bacterial Proteins/genetics/metabolism Base Sequence Chromosome Deletion Fungal Proteins/*genetics/metabolism *Gene Expression Regulation, Fungal *Homeodomain Proteins Mating Factor Models, Genetic Molecular Sequence Data Oligo;"
Notes:		"MedlineSengupta, P Cochran, B H eng R01-GM44821/GM/NIGMS NIH HHS/ Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S. 1991/10/01 Genes Dev. 1991 Oct; 5(10):1924-34. doi: 10.1101/gad.5.10.1924"