| Title: | HSPRO controls early Nicotiana attenuata seedling growth during interaction with the fungus Piriformospora indica |
| Author(s): | Schuck S; Camehl I; Gilardoni PA; Oelmueller R; Baldwin IT; Bonaventure G; |
| Address: | "Department of Molecular Ecology, Max Planck Institute for Chemical Ecology, Jena 07745, Germany" |
| ISSN/ISBN: | 1532-2548 (Electronic) 0032-0889 (Print) 0032-0889 (Linking) |
| Abstract: | "In a previous study aimed at identifying regulators of Nicotiana attenuata responses against chewing insects, a 26-nucleotide tag matching the HSPRO (ORTHOLOG OF SUGAR BEET Hs1(pro)(-)(1)) gene was found to be strongly induced after simulated herbivory (Gilardoni et al., 2010). Here we characterized the function of HSPRO during biotic interactions in transgenic N. attenuata plants silenced in its expression (ir-hspro). In wild-type plants, HSPRO expression was not only induced during simulated herbivory but also when leaves were inoculated with Pseudomonas syringae pv tomato DC3000 and roots with the growth-promoting fungus Piriformospora indica. Reduced HSPRO expression did not affect the regulation of direct defenses against Manduca sexta herbivory or P. syringae pv tomato DC3000 infection rates. However, reduced HSPRO expression positively influenced early seedling growth during interaction with P. indica; fungus-colonized ir-hspro seedlings increased their fresh biomass by 30% compared with the wild type. Grafting experiments demonstrated that reduced HSPRO expression in roots was sufficient to induce differential growth promotion in both roots and shoots. This effect was accompanied by changes in the expression of 417 genes in colonized roots, most of which were metabolic genes. The lack of major differences in the metabolic profiles of ir-hspro and wild-type colonized roots (as analyzed by liquid chromatography time-of-flight mass spectrometry) suggested that accelerated metabolic rates were involved. We conclude that HSPRO participates in a whole-plant change in growth physiology when seedlings interact with P. indica" |
| Keywords: | "Agrobacterium tumefaciens/genetics/metabolism Animals Basidiomycota/*physiology Cell Death Gene Expression Profiling Gene Expression Regulation, Plant Gene Silencing Genes, Plant Herbivory Manduca Metabolome Plant Diseases/microbiology Plant Growth Regula;" |
| Notes: | "MedlineSchuck, Stefan Camehl, Iris Gilardoni, Paola A Oelmueller, Ralf Baldwin, Ian T Bonaventure, Gustavo eng Research Support, Non-U.S. Gov't 2012/08/16 Plant Physiol. 2012 Oct; 160(2):929-43. doi: 10.1104/pp.112.203976. Epub 2012 Aug 14" |
