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Phytochemistry

Title:		Cloning and characterization of isoprenyl diphosphate synthases with farnesyl diphosphate and geranylgeranyl diphosphate synthase activity from Norway spruce (Picea abies) and their relation to induced oleoresin formation
Author(s):		Schmidt A; Gershenzon J;
Address:		"Max Planck Institute for Chemical Ecology, Department of Biochemistry, Hans-Knoll-Str. 8, D-07745 Jena, Germany. aschmidt@ice.mpg.de"
Journal Title:		Phytochemistry
Year:		2007
Volume:		20070710
Issue:		21
Page Number:		2649 - 2659
DOI:		10.1016/j.phytochem.2007.05.037
ISSN/ISBN:		0031-9422 (Print) 0031-9422 (Linking)
Abstract:		"The conifer Picea abies (Norway spruce) employs terpenoid-based oleoresins as part of its constitutive and induced defense responses to herbivores and pathogens. The isoprenyl diphosphate synthases are branch-point enzymes of terpenoid biosynthesis leading to the various terpene classes. We isolated three genes encoding isoprenyl diphosphate synthases from P. abies cDNA libraries prepared from the bark and wood of methyl jasmonate-treated saplings and screened via a homology-based PCR approach using degenerate primers. Enzyme assays of the purified recombinant proteins expressed in Escherichia coli demonstrated that one gene (PaIDS 4) encodes a farnesyl diphosphate synthase and the other two (PaIDS 5 and PaIDS 6) encode geranylgeranyl diphosphate synthases. The sequences have moderate similarity to those of farnesyl diphosphate and geranylgeranyl diphosphate synthases already known from plants, and the kinetic properties of the enzymes are not unlike those of other isoprenyl diphosphate synthases. Of the three genes, only PaIDS 5 displayed a significant increase in transcript level in response to methyl jasmonate spraying, suggesting its involvement in induced oleoresin biosynthesis"
Keywords:		"Alkyl and Aryl Transferases/*chemistry/genetics/*metabolism Amino Acid Sequence Cloning, Molecular Diterpenes Kinetics Molecular Sequence Data Phylogeny Picea/*enzymology/genetics Plant Extracts/*biosynthesis Polyisoprenyl Phosphates/*biosynthesis Protein;"
Notes:		"MedlineSchmidt, Axel Gershenzon, Jonathan eng Research Support, Non-U.S. Gov't England 2007/07/13 Phytochemistry. 2007 Nov; 68(21):2649-59. doi: 10.1016/j.phytochem.2007.05.037. Epub 2007 Jul 10"