| Title: | "Host Tissue Environment Directs Activities of an Epichloe Endophyte, While It Induces Systemic Hormone and Defense Responses in Its Native Perennial Ryegrass Host" |
| Author(s): | Schmid J; Day R; Zhang N; Dupont PY; Cox MP; Schardl CL; Minards N; Truglio M; Moore N; Harris DR; Zhou Y; |
| Address: | "1 Institute of Fundamental Sciences, Massey University, Palmerston North 4410, New Zealand. 2 School of Medical Sciences, University of Otago, Dunedin 9054, New Zealand. 3 Department of Plant Pathology, University of Kentucky, Lexington 40546-0312, U.S.A. 4 Manawatu Microscopy and Imaging Centre, Palmerston North 4410, New Zealand. 5 Computer Science Department, University of Kentucky; and. 6 Institute for Pharmaceutical Outcomes & Policy, University of Kentucky" |
| Journal Title: | Mol Plant Microbe Interact |
| DOI: | 10.1094/MPMI-10-16-0215-R |
| ISSN/ISBN: | 0894-0282 (Print) 0894-0282 (Linking) |
| Abstract: | "Increased resilience of pasture grasses mediated by fungal Epichloe endophytes is crucial to pastoral industries. The underlying mechanisms are only partially understood and likely involve very different activities of the endophyte in different plant tissues and responses of the plant to these. We analyzed the transcriptomes of Epichloe festucae and its host, Lolium perenne, in host tissues of different function and developmental stages. The endophyte contributed approximately 10x more to the transcriptomes than to the biomass of infected tissues. Proliferating mycelium in growing host tissues highly expressed genes involved in hyphal growth. Nonproliferating mycelium in mature plant tissues, transcriptionally equally active, highly expressed genes involved in synthesizing antiherbivore compounds. Transcripts from the latter accounted for 4% of fungal transcripts. Endophyte infection systemically but moderately increased transcription of L. perenne genes with roles in hormone biosynthesis and perception as well as stress and pathogen resistance while reducing expression of genes involved in photosynthesis. There was a good correlation between transcriptome-based observations and physiological observations. Our data indicate that the fitness-enhancing effects of the endophyte are based both on its biosynthetic activities, predominantly in mature host tissues, and also on systemic alteration of the host's hormonal responses and induction of stress response genes. [Formula: see text] Copyright (c) 2017 The Author(s). This is an open access article distributed under the CC BY-NC-ND 4.0 International license" |
| Keywords: | "Cell Nucleus/metabolism Chloroplasts/metabolism DNA, Plant/metabolism Endophytes/genetics/*physiology *Environment Epichloe/genetics/*physiology Gene Expression Profiling Gene Expression Regulation, Plant Genes, Fungal Herbivory *Host-Pathogen Interaction;" |
| Notes: | "MedlineSchmid, Jan Day, Robert Zhang, Ningxin Dupont, Pierre-Yves Cox, Murray P Schardl, Christopher L Minards, Niki Truglio, Mauro Moore, Neil Harris, Daniel R Zhou, Yanfei eng Research Support, Non-U.S. Gov't 2016/12/28 Mol Plant Microbe Interact. 2017 Feb; 30(2):138-149. doi: 10.1094/MPMI-10-16-0215-R. Epub 2017 Mar 3" |
