| Title: | Detection and evaluation of volatile and non-volatile antifungal compounds produced by Bacillus spp. strains |
| Author(s): | Santos JBD; Cruz JO; Geraldo LC; Dias EG; Queiroz PRM; Monnerat RG; Borges M; Blassioli-Moraes MC; Blum LEB; |
| Address: | "University of Brasilia (UnB), Postgraduate Program in Agronomy, Faculty of Agronomy and Veterinary Medicine, Brasilia 70910-900, Brazil. Electronic address: jonatas.bsantos@hotmail.com. University of Brasilia (UnB), Postgraduate Program in Agronomy, Faculty of Agronomy and Veterinary Medicine, Brasilia 70910-900, Brazil. University Center of Brasilia (UNICEUB), Brasilia 70790-075, Brazil. Empresa Brasileira de Pesquisa Agropecuaria (Brazilian Agricultural Research Corporation) Genetic Resources and Biotechnology (CENARGEN), Brasilia 70770-917, Brazil" |
| DOI: | 10.1016/j.micres.2023.127465 |
| ISSN/ISBN: | 1618-0623 (Electronic) 0944-5013 (Linking) |
| Abstract: | "The identification of antifungal compounds produced by microorganisms is crucial in the context of sustainable agriculture. Bacteria of the genus Bacillus have a broad spectrum of action that can influence plant growth and control pests, vectors of public health relevance and phytopathogens. Lipopeptides are the main compounds related to the biological control of several pathogen species. Strains with biotechnological potential are identified by means of in vitro bioassays and molecular tests. In this study, strains from the Bacillus Bank of Brazilian Agricultural Research Corporation (EMBRAPA/DF/Brazil) were selected to control the fungal pathogens Sclerotinia sclerotiorum and Fusarium oxysporum by pairing assays. The detection of genes for biosynthesis of antifungal compounds from strains with high pathogen-inhibition capacity was correlated with peptide synthesis, such as bacillomycin D, fengycin d, bacilysin and surfactin. Their gene expression in contact with the pathogen was analyzed by Real-Time PCR. The volatile organic compounds produced by selected Bacillus strains were identified and quantified. In co-culture assays, the inhibition zone between Bacillus strains and Sclerotinia sclerotiorum was evaluated by scanning electron microscopy. Thirteen potentially anti-pathogenic strains were selected. Genes related to the synthesis of antifungal peptides were detected in 11 of them. In five strains, all tested genes were detected. Bacillomycin was the most frequently found lipopeptide gene. The fungus-bacteria interaction potentiated the production of volatiles. Several ketones and other volatile compounds with antifungal activity were identified. Relevant morphological changes in the fungus were observed when paired with bacteria. The study demonstrated the efficacy of the selected strains with regard to the biological control of phytopathogens and their biotechnological potential" |
| Keywords: | Antifungal Agents/pharmacology/metabolism *Bacillus/metabolism *Ascomycota Lipopeptides/metabolism Bacteria/metabolism Plant Diseases/microbiology Biological control Sclerotinia sclerotiorum Secondary metabolites Volatile organic compounds; |
| Notes: | "MedlineSantos, Jonatas Barros Dos Cruz, Jose de Oliveira Geraldo, Leticia Costa Dias, Emanuel Guimaraes Queiroz, Paulo Roberto Martins Monnerat, Rose Gomes Borges, Miguel Blassioli-Moraes, Maria Carolina Blum, Luiz Eduardo Bassay eng Germany 2023/08/06 Microbiol Res. 2023 Oct; 275:127465. doi: 10.1016/j.micres.2023.127465. Epub 2023 Jul 29" |
