| Title: | MALDI-TOF MS for the Identification of Cultivable Organic-Degrading Bacteria in Contaminated Groundwater near Unconventional Natural Gas Extraction Sites |
| Author(s): | Santos IC; Martin MS; Carlton DD; Amorim CL; Castro PML; Hildenbrand ZL; Schug KA; |
| Address: | "Department of Chemistry and Biochemistry, The University of Texas at Arlington, Arlington, TX 76019-0065, USA. ines.santos@uta.edu. Affiliate of the Collaborative Laboratories for Environmental Analysis and Remediation, The University of Texas at Arlington, Arlington, TX 76019-0065, USA. ines.santos@uta.edu. Department of Chemistry and Biochemistry, The University of Texas at Arlington, Arlington, TX 76019-0065, USA. mistysmartin@mavs.uta.edu. Department of Chemistry and Biochemistry, The University of Texas at Arlington, Arlington, TX 76019-0065, USA. doug.carlton@uta.edu. Affiliate of the Collaborative Laboratories for Environmental Analysis and Remediation, The University of Texas at Arlington, Arlington, TX 76019-0065, USA. doug.carlton@uta.edu. CBQF-Centro de Biotecnologia e Quimica Fina-Laboratorio Associado, Escola Superior de Biotecnologia, Universidade Catolica Portuguesa/Porto, Rua Arquiteto Lobao Vital, Apartado 2511, 4202-401 Porto, Portugal. camorim@porto.ucp.pt. Biology Department and CESAM, University of Aveiro, Campus Universitario de Santiago, 3810-193 Aveiro, Portugal. camorim@porto.ucp.pt. CBQF-Centro de Biotecnologia e Quimica Fina-Laboratorio Associado, Escola Superior de Biotecnologia, Universidade Catolica Portuguesa/Porto, Rua Arquiteto Lobao Vital, Apartado 2511, 4202-401 Porto, Portugal. plcastro@porto.ucp.pt. Affiliate of the Collaborative Laboratories for Environmental Analysis and Remediation, The University of Texas at Arlington, Arlington, TX 76019-0065, USA. kschug@uta.edu. Inform Environmental, LLC, Dallas, TX 75206, USA. kschug@uta.edu. Department of Chemistry and Biochemistry, The University of Texas at Arlington, Arlington, TX 76019-0065, USA. zac@informenv.com. Affiliate of the Collaborative Laboratories for Environmental Analysis and Remediation, The University of Texas at Arlington, Arlington, TX 76019-0065, USA. zac@informenv.com" |
| DOI: | 10.3390/microorganisms5030047 |
| ISSN/ISBN: | 2076-2607 (Print) 2076-2607 (Electronic) 2076-2607 (Linking) |
| Abstract: | "Groundwater quality and quantity is of extreme importance as it is a source of drinking water in the United States. One major concern has emerged due to the possible contamination of groundwater from unconventional oil and natural gas extraction activities. Recent studies have been performed to understand if these activities are causing groundwater contamination, particularly with respect to exogenous hydrocarbons and volatile organic compounds. The impact of contaminants on microbial ecology is an area to be explored as alternatives for water treatment are necessary. In this work, we identified cultivable organic-degrading bacteria in groundwater in close proximity to unconventional natural gas extraction. Pseudomonas stutzeri and Acinetobacter haemolyticus were identified using matrix-assisted laser desorption/ionization-time-of-flight-mass spectrometry (MALDI-TOF MS), which proved to be a simple, fast, and reliable method. Additionally, the potential use of the identified bacteria in water and/or wastewater bioremediation was studied by determining the ability of these microorganisms to degrade toluene and chloroform. In fact, these bacteria can be potentially applied for in situ bioremediation of contaminated water and wastewater treatment, as they were able to degrade both compounds" |
| Keywords: | bioremediation chloroform groundwater hydraulic fracturing mass spectrometry microorganism identification toluene; |
| Notes: | "PubMed-not-MEDLINESantos, Ines C Martin, Misty S Carlton, Doug D Amorim, Catarina L Castro, Paula M L Hildenbrand, Zacariah L Schug, Kevin A eng Switzerland 2017/08/11 Microorganisms. 2017 Aug 10; 5(3):47. doi: 10.3390/microorganisms5030047" |
