| Title: | Nonhuman primate breath volatile organic compounds associate with developmental programming and cardio-metabolic status |
| Author(s): | Bishop AC; Libardoni M; Choudary A; Misra B; Lange K; Bernal J; Nijland M; Li C; Olivier M; Nathanielsz PW; Cox LA; |
| Address: | "Department of Genetics, Texas Biomedical Research Institute, San Antonio, TX, United States of America" |
| ISSN/ISBN: | 1752-7163 (Electronic) 1752-7155 (Print) 1752-7155 (Linking) |
| Abstract: | "Rodent and nonhuman primate studies indicate that developmental programming by reduced perinatal nutrition negatively impacts life course cardio-metabolic health. We have developed a baboon model in which we feed control mothers (CON) ad libitum while nutrient restricted mothers are fed 70% of ad libitum global feed in pregnancy and lactation. Offspring of nutrient restricted mothers are intrauterine growth restricted (IUGR) at term. By 3.5 years IUGR baboons showed signs of insulin resistance, indicating a pre-diabetic phenotype, in contrast to healthy CON offspring. We hypothesized that a novel breath analysis approach would provide markers of the altered cardio-metabolic state in a non-invasive manner. Here we assess whether exhaled breath volatile organic compounds (VOCs) collected from this unique cohort of juvenile baboons with documented cardio-metabolic dysfunction resulting from in utero programming can be detected from their breath signatures. Breath was collected from male and female CON and IUGR baboons at 4.8 +/- 0.2 years (human equivalent approximately 13 years). Breath VOCs were quantified using a two-dimensional gas chromatography mass spectrometer. Two-way ANOVA, on 76 biologically relevant VOCs identified 27 VOCs (p < 0.05) with altered abundances between groups (sex, birthweight, and sex x birthweight). The 27 VOCs included 2-pentanone, 2-octanone, 2,2,7,7-tetramethyloctane and 3-methyl-1-heptene, which have not previously been associated with cardio-metabolic disease. Unsupervised principal component analysis of these VOCs could discriminate the four clusters defining males, females, CON and IUGR. This study, which is the first to assess quantifiable breath signatures associated with cardio-metabolic programing for any model of IUGR, demonstrates the translational value of this unique model to identify metabolites of programmed cardio-metabolic dysfunction in breath signatures. Future studies are required to validate the translatability of these findings to humans" |
| Keywords: | Animals Biomarkers/metabolism Birth Weight Breath Tests/*methods Cardiovascular System/*metabolism Exhalation Female Fetal Growth Retardation/diagnosis Gas Chromatography-Mass Spectrometry Male Papio Pregnancy Principal Component Analysis Volatile Organic; |
| Notes: | "MedlineBishop, Andrew C Libardoni, Mark Choudary, Ahsan Misra, Biswapriya Lange, Kenneth Bernal, John Nijland, Mark Li, Cun Olivier, Michael Nathanielsz, Peter W Cox, Laura A eng C06 RR017515/RR/NCRR NIH HHS/ C06 RR013556/RR/NCRR NIH HHS/ P51 OD011133/OD/NIH HHS/ C06 RR014578/RR/NCRR NIH HHS/ C06 RR015456/RR/NCRR NIH HHS/ P51 RR013986/RR/NCRR NIH HHS/ Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't England 2018/03/30 J Breath Res. 2018 May 14; 12(3):036016. doi: 10.1088/1752-7163/aaba84" |
