« Previous AbstractIndoor ozone/human chemistry and ventilation strategies    Next AbstractHomologous and heterologous expression of grapevine E-(beta)-caryophyllene synthase (VvGwECar2) »

Methods Mol Biol

Title:		Natural Transformation of Oral Streptococci by Use of Synthetic Pheromones
Author(s):		Salvadori G; Junges R; Khan R; Amdal HA; Morrison DA; Petersen FC;
Address:		"Department of Oral Biology, Faculty of Dentistry, University of Oslo, P.O. Box 1052, Blindern, Oslo, 0316, Norway. Department of Biological Sciences, College of Liberal Arts and Sciences, University of Illinois at Chicago, Chicago, IL, USA. Department of Oral Biology, Faculty of Dentistry, University of Oslo, P.O. Box 1052, Blindern, Oslo, 0316, Norway. f.c.petersen@odont.uio.no"
Journal Title:		Methods Mol Biol
Year:		2017
Volume:		1537
Issue:
Page Number:		219 - 232
DOI:		10.1007/978-1-4939-6685-1_13
ISSN/ISBN:		1940-6029 (Electronic) 1064-3745 (Linking)
Abstract:		"The discovery that Streptococcus pneumoniae uses a competence-stimulating peptide (CSP) to induce competence for natural transformation, and that other species of the mitis and the anginosus streptococcal groups use a similar system, has expanded the tools to explore gene function and regulatory pathways in streptococci. Two other classes of pheromones have been discovered since then, comprising the bacteriocin-inducing peptide class found in Streptococcus mutans (also named CSP, although different from the former) and the SigX-inducing peptides (XIP), in the mutans, salivarius, bovis, and pyogenes groups of streptococci. The three classes of peptide pheromones can be ordered from peptide synthesis services at affordable prices, and used in transformation assays to obtain competent cultures consistently at levels usually higher than those achieved during spontaneous competence. In this chapter, we present protocols for natural transformation of oral streptococci that are based on the use of synthetic pheromones, with examples of conditions optimized for transformation of S. mutans and Streptococcus mitis"
Keywords:		"Bacterial Proteins/metabolism/pharmacology Humans Mouth/*microbiology Peptides/pharmacology Pheromones/chemical synthesis/*pharmacology Streptococcus/*drug effects/*physiology Streptococcus mutans/drug effects/physiology Transformation, Bacterial/*drug ef;"
Notes:		"MedlineSalvadori, Gabriela Junges, Roger Khan, Rabia Amdal, Heidi A Morrison, Donald A Petersen, Fernanda C eng Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. 2016/12/08 Methods Mol Biol. 2017; 1537:219-232. doi: 10.1007/978-1-4939-6685-1_13"