| Title: | Isolation and Identification of Wild Yeast from Malaysian Grapevine and Evaluation of Their Potential Antimicrobial Activity against Grapevine Fungal Pathogens |
| Author(s): | Sabaghian S; Braschi G; Vannini L; Patrignani F; Samsulrizal NH; Lanciotti R; |
| Address: | "Department of Agricultural and Food Sciences, University of Bologna, Piazza Goidanich 60, 47521 Cesena, Italy. Interdepartmental Center for Industrial Agri-Food Research, University of Bologna, Piazza Goidanich 60, 47521 Cesena, Italy. Department of Plant Science, Kulliyyah of Science, International Islamic University Malaysia, Kuantan Campus, Kuantan 25200, Malaysia" |
| DOI: | 10.3390/microorganisms9122582 |
| ISSN/ISBN: | 2076-2607 (Print) 2076-2607 (Electronic) 2076-2607 (Linking) |
| Abstract: | "Pathogenic fungi belonging to the genera Botrytis, Phaeomoniella, Fusarium, Alternaria and Aspergillus are responsible for vines diseases that affect the growth, grapevine yield and organoleptic quality. Among innovative strategies for in-field plant disease control, one of the most promising is represented by biocontrol agents, including wild epiphytic yeast strains of grapevine berries. Twenty wild yeast, isolated and molecularly identified from three different Malaysian regions (Perlis, Perak and Pahang), were evaluated in a preliminary screening test on agar to select isolates with inhibition against Botrytis cinerea. On the basis of the results, nine yeasts belonging to genera Hanseniaspora, Starmerella, Metschnikowia, Candida were selected and then tested against five grape berry pathogens: Aspergillus carbonarius, Aspergillus ochraceus, Fusarium oxysporum, Alternaria alternata and Phaeomoniella chlamydospora.Starmerella bacillaris FE08.05 and Metschnikowia pulcherrima GP8 and Hanseniaspora uvarum GM19 showed the highest effect on inhibiting mycelial growth, which ranged between 15.1 and 4.3 mm for the inhibition ring. The quantitative analysis of the volatile organic compound profiles highlighted the presence of isoamyl and phenylethyl alcohols and an overall higher presence of low-chain fatty acids and volatile ethyl esters. The results of this study suggest that antagonist yeasts, potentially effective for the biological control of pathogenic moulds, can be found among the epiphytic microbiota associated with grape berries" |
| Keywords: | Metschnikowia pulcherrima Starmerella bacillaris antifungal activity biocontrol agents epiphytic yeast grapevines plant pathogens volatile organic compounds; |
| Notes: | "PubMed-not-MEDLINESabaghian, Simin Braschi, Giacomo Vannini, Lucia Patrignani, Francesca Samsulrizal, Nurul Hidayah Lanciotti, Rosalba eng Switzerland 2021/12/25 Microorganisms. 2021 Dec 13; 9(12):2582. doi: 10.3390/microorganisms9122582" |
