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Methods Mol Biol

Title:		Analysis of volatile compounds emitted by filamentous fungi using solid-phase microextraction-gas chromatography/mass spectrometry
Author(s):		Roze LV; Beaudry RM; Linz JE;
Address:		"Department of Food Science and Human Nutrition, Michigan State University, East Lansing, MI, USA. roze@msu.edu"
Journal Title:		Methods Mol Biol
Year:		2012
Volume:		944
Issue:
Page Number:		133 - 142
DOI:		10.1007/978-1-62703-122-6_9
ISSN/ISBN:		1940-6029 (Electronic) 1064-3745 (Linking)
Abstract:		"Here, we describe a solid-phase microextraction-gas chromatography/mass spectrometry (SPME-GC/MS) analytical approach that identifies and analyzes volatile compounds in the headspace above a live fungal culture. This approach is a sensitive, solvent-free, robust technique; most importantly from a practical standpoint, this approach is noninvasive and requires minimal sample handling. Aliquots of liquid fungal cultures are placed into vials equipped with inert septa and equilibrated at a constant temperature, and headspace gases are sampled using an SPME fiber inserted through the septum into the headspace above the fungal culture for a standardized period of time. The outer polymer coating of a fused silica fiber absorbs volatiles from the headspace; the volatiles are then desorbed in the hot GC inlet and chromatographed in the usual manner. The separated compounds are subsequently identified by mass spectrometry. All steps in volatile profiling of a single sample from volatile sorption on a fiber to obtaining a list of volatiles can take as little as 15 min or can be extended to several hours if longer sorption is required for compounds present at very low levels and/or have low rates of diffusion"
Keywords:		"Aspergillus/*metabolism Gas Chromatography-Mass Spectrometry/instrumentation/*methods Software Solid Phase Microextraction/instrumentation/*methods Spores, Fungal/metabolism Time Factors Volatile Organic Compounds/*analysis/*isolation & purification/metab;"
Notes:		"MedlineRoze, Ludmila V Beaudry, Randolph M Linz, John E eng CA52003-18/CA/NCI NIH HHS/ Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't 2012/10/16 Methods Mol Biol. 2012; 944:133-42. doi: 10.1007/978-1-62703-122-6_9"