| Title: | Farnesol-detecting olfactory neurons in Drosophila |
| Author(s): | Ronderos DS; Lin CC; Potter CJ; Smith DP; |
| Address: | "Departments of Pharmacology and Neuroscience, University of Texas Southwestern Medical Center, Dallas, Texas 75390, and Solomon H. Snyder Department of Neuroscience, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205" |
| DOI: | 10.1523/JNEUROSCI.4582-13.2014 |
| ISSN/ISBN: | 1529-2401 (Electronic) 0270-6474 (Print) 0270-6474 (Linking) |
| Abstract: | "We set out to deorphanize a subset of putative Drosophila odorant receptors expressed in trichoid sensilla using a transgenic in vivo misexpression approach. We identified farnesol as a potent and specific activator for the orphan odorant receptor Or83c. Farnesol is an intermediate in juvenile hormone biosynthesis, but is also produced by ripe citrus fruit peels. Here, we show that farnesol stimulates robust activation of Or83c-expressing olfactory neurons, even at high dilutions. The CD36 homolog Snmp1 is required for normal farnesol response kinetics. The neurons expressing Or83c are found in a subset of poorly characterized intermediate sensilla. We show that these neurons mediate attraction behavior to low concentrations of farnesol and that Or83c receptor mutants are defective for this behavior. Or83c neurons innervate the DC3 glomerulus in the antennal lobe and projection neurons relaying information from this glomerulus to higher brain centers target a region of the lateral horn previously implicated in pheromone perception. Our findings identify a sensitive, narrowly tuned receptor that mediates attraction behavior to farnesol and demonstrates an effective approach to deorphanizing odorant receptors expressed in neurons located in intermediate and trichoid sensilla that may not function in the classical 'empty basiconic neuron' system" |
| Keywords: | "Animals Animals, Genetically Modified Behavior, Animal/physiology Drosophila Proteins/genetics/*physiology Drosophila melanogaster/anatomy & histology/*physiology *Farnesol Female Ganglia, Invertebrate/cytology/physiology Male Membrane Proteins/physiology;neuroscience;" |
| Notes: | "MedlineRonderos, David S Lin, Chun-Chieh Potter, Christopher J Smith, Dean P eng R01 DC011751/DC/NIDCD NIH HHS/ R01 DCD02539/PHS HHS/ P30DC005211/DC/NIDCD NIH HHS/ R01 DC013079/DC/NIDCD NIH HHS/ R01 DC015230/DC/NIDCD NIH HHS/ P30 DC005211/DC/NIDCD NIH HHS/ R01 DC01307901/DC/NIDCD NIH HHS/ R01 DCD011751/PHS HHS/ Research Support, N.I.H., Extramural 2014/03/14 J Neurosci. 2014 Mar 12; 34(11):3959-68. doi: 10.1523/JNEUROSCI.4582-13.2014" |
