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Animal

Title:		Evaluating the effect of phenolic compounds as hydrogen acceptors when ruminal methanogenesis is inhibited in vitro - Part 2. Dairy goats
Author(s):		Romero P; Huang R; Jimenez E; Palma-Hidalgo JM; Ungerfeld EM; Popova M; Morgavi DP; Belanche A; Yanez-Ruiz DR;
Address:		"Estacion Experimental del Zaidin (CSIC), Profesor Albareda, 1, 18008 Granada, Spain. Universite Clermont Auvergne, INRAE, VetAgro Sup, UMR Herbivores, F-63122 Saint-Genes Champanelle, France. Centro Regional de Investigacion Carillanca, Instituto de Investigaciones Agropecuarias INIA, Temuco 4880000, Chile. Estacion Experimental del Zaidin (CSIC), Profesor Albareda, 1, 18008 Granada, Spain; Departamento de Produccion Animal y Ciencia de los Alimentos, Universidad de Zaragoza, Miguel Servet 177, 50013 Zaragoza, Spain. Estacion Experimental del Zaidin (CSIC), Profesor Albareda, 1, 18008 Granada, Spain. Electronic address: david.yanez@eez.csic.es"
Journal Title:		Animal
Year:		2023
Volume:		20230329
Issue:		5
Page Number:		100789 -
DOI:		10.1016/j.animal.2023.100789
ISSN/ISBN:		1751-732X (Electronic) 1751-7311 (Linking)
Abstract:		"Most mitigation strategies to reduce enteric methane (CH(4)) production in the rumen induce an excess of rumen dihydrogen (H(2)) that is expelled and consequently not redirected to the synthesis of metabolites that can be utilised by the ruminant. We hypothesised that phenolic compounds can be potential H(2) acceptors when added to the diet, as they can be degraded to compounds that may be beneficial for the animal, using part of the H(2) available when ruminal methanogenesis is inhibited. We performed four in vitro incubation experiments using rumen inoculum from Murciano-Granadina adult goats: Experiment 1 examined the inhibitory potential of Asparagopsis taxiformis (AT) at different concentrations (0, 1, 2, 3, 4 and 5% of the substrate on a DM basis) in 24 h incubations; Experiment 2 investigated the effect of a wide range of phenolic compounds (phenol, catechol, resorcinol, hydroquinone, pyrogallol, phloroglucinol, gallic acid and formic acid) at different doses (0, 2, 4, and 6 mM) on rumen fermentation for 24 h; Experiment 3 evaluated the combined effect of each phenolic compound at 6 mM with AT at 2% DM in sequential batch cultures for 5 days; and Experiment 4 examined the dose-response effect of phloroglucinol at different concentrations (0, 6, 16, 26 and 36 mM) combined with AT in sequential batch cultures for 5 days. Results from Experiment 1 confirmed that AT at 2% DM substantially inhibited CH(4) production while significantly increasing H(2) accumulation and decreasing the acetate:propionate ratio. Results from Experiment 2 showed that phenolic compounds did not negatively affect rumen fermentation at any dose. In Experiment 3, each phenolic compound at 6 mM combined with AT at 2% DM inhibited CH(4) production. Phloroglucinol numerically decreased H(2) accumulation and significantly increased total gas production (TGP), volatile fatty acid (VFA) production and the acetate:propionate ratio. In Experiment 4, phloroglucinol at increasing doses supplemented with AT at 2% DM significantly decreased H(2) accumulation and the abundances of archaea, protozoa and fungi abundances, and increased TGP, total VFA production and the acetate:propionate ratio in a dose-dependent way. In conclusion, combined treatment with AT and phloroglucinol was successful to mitigate CH(4) production while preventing the accumulation of H(2), leading to an increase in acetate and total VFA production and therefore an improvement in rumen fermentation in goats"
Keywords:		"Animals *Propionates/pharmacology/metabolism *Hydrogen/metabolism Rumen/metabolism Animal Feed/analysis Diet/veterinary Fatty Acids, Volatile/metabolism Acetates Phenols/pharmacology Goats/metabolism Phloroglucinol/metabolism Fermentation Methane/metaboli;"
Notes:		"MedlineRomero, P Huang, R Jimenez, E Palma-Hidalgo, J M Ungerfeld, E M Popova, M Morgavi, D P Belanche, A Yanez-Ruiz, D R eng England 2023/04/24 Animal. 2023 May; 17(5):100789. doi: 10.1016/j.animal.2023.100789. Epub 2023 Mar 29"