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Microb Cell Fact

Title:		Key role of lipid management in nitrogen and aroma metabolism in an evolved wine yeast strain
Author(s):		Rollero S; Mouret JR; Sanchez I; Camarasa C; Ortiz-Julien A; Sablayrolles JM; Dequin S;
Address:		"INRA, UMR1083, 34060, Montpellier, France. rollero@supagro.inra.fr. SupAgro, UMR1083, 34060, Montpellier, France. rollero@supagro.inra.fr. Universite Montpellier, UMR1083, 34060, Montpellier, France. rollero@supagro.inra.fr. Lallemand SAS, 31700, Blagnac, France. rollero@supagro.inra.fr. INRA, UMR1083, 34060, Montpellier, France. mouretj@supagro.inra.fr. SupAgro, UMR1083, 34060, Montpellier, France. mouretj@supagro.inra.fr. Universite Montpellier, UMR1083, 34060, Montpellier, France. mouretj@supagro.inra.fr. INRA, UMR1083, 34060, Montpellier, France. sanchezi@supagro.inra.fr. SupAgro, UMR1083, 34060, Montpellier, France. sanchezi@supagro.inra.fr. Universite Montpellier, UMR1083, 34060, Montpellier, France. sanchezi@supagro.inra.fr. INRA, UMR1083, 34060, Montpellier, France. camarasa@supagro.inra.fr. SupAgro, UMR1083, 34060, Montpellier, France. camarasa@supagro.inra.fr. Universite Montpellier, UMR1083, 34060, Montpellier, France. camarasa@supagro.inra.fr. Lallemand SAS, 31700, Blagnac, France. ajulien@lallemand.com. INRA, UMR1083, 34060, Montpellier, France. sablayro@supagro.inra.fr. SupAgro, UMR1083, 34060, Montpellier, France. sablayro@supagro.inra.fr. Universite Montpellier, UMR1083, 34060, Montpellier, France. sablayro@supagro.inra.fr. INRA, UMR1083, 34060, Montpellier, France. dequin@supagro.inra.fr. SupAgro, UMR1083, 34060, Montpellier, France. dequin@supagro.inra.fr. Universite Montpellier, UMR1083, 34060, Montpellier, France. dequin@supagro.inra.fr"
Journal Title:		Microb Cell Fact
Year:		2016
Volume:		20160209
Issue:
Page Number:		32 -
DOI:		10.1186/s12934-016-0434-6
ISSN/ISBN:		1475-2859 (Electronic) 1475-2859 (Linking)
Abstract:		"BACKGROUND: Fermentative aromas play a key role in the organoleptic profile of young wines. Their production depends both on yeast strain and fermentation conditions. A present-day trend in the wine industry consists in developing new strains with aromatic properties using adaptive evolution approaches. An evolved strain, Affinity ECA5, overproducing esters, was recently obtained. In this study, dynamics of nitrogen consumption and of the fermentative aroma synthesis of the evolved and its ancestral strains were compared and coupled with a transcriptomic analysis approach to better understand the metabolic reshaping of Affinity ECA5. RESULTS: Nitrogen assimilation was different between the two strains, particularly amino acids transported by carriers regulated by nitrogen catabolite repression. We also observed differences in the kinetics of fermentative aroma production, especially in the bioconversion of higher alcohols into acetate esters. Finally, transcriptomic data showed that the enhanced bioconversion into acetate esters by the evolved strain was associated with the repression of genes involved in sterol biosynthesis rather than an enhanced expression of ATF1 and ATF2 (genes coding for the enzymes responsible for the synthesis of acetate esters from higher alcohols). CONCLUSIONS: An integrated approach to yeast metabolism-combining transcriptomic analyses and online monitoring data-showed differences between the two strains at different levels. Differences in nitrogen source consumption were observed suggesting modifications of NCR in the evolved strain. Moreover, the evolved strain showed a different way of managing the lipid source, which notably affected the production of acetate esters, likely because of a greater availability of acetyl-CoA for the evolved strain"
Keywords:		"Fermentation/drug effects Gene Expression Regulation, Fungal/drug effects Lipid Metabolism/*drug effects/genetics Nitrogen/metabolism/*pharmacology *Odorants Pentanols/metabolism Principal Component Analysis Saccharomyces cerevisiae/genetics/*metabolism T;"
Notes:		"MedlineRollero, Stephanie Mouret, Jean-Roch Sanchez, Isabelle Camarasa, Carole Ortiz-Julien, Anne Sablayrolles, Jean-Marie Dequin, Sylvie eng Research Support, Non-U.S. Gov't England 2016/02/11 Microb Cell Fact. 2016 Feb 9; 15:32. doi: 10.1186/s12934-016-0434-6"