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Comp Biochem Physiol Part D Genomics Proteomics

Title:		Analysis of the grapevine moth Lobesia botrana antennal transcriptome and expression of odorant-binding and chemosensory proteins
Author(s):		Rojas V; Jimenez H; Palma-Millanao R; Gonzalez-Gonzalez A; Machuca J; Godoy R; Ceballos R; Mutis A; Venthur H;
Address:		"Carrera Bioquimica, Departamento de Ciencias Quimicas y Recursos Naturales, Universidad de La Frontera, Temuco, Chile; Laboratorio de Quimica Ecologica, Departamento de Ciencias Quimicas y Recursos Naturales, Universidad de La Frontera, Temuco, Chile. Laboratorio de Quimica Ecologica, Departamento de Ciencias Quimicas y Recursos Naturales, Universidad de La Frontera, Temuco, Chile. Instituto de Ciencias Biologicas, Universidad de Talca, Talca, Chile; Millennium Nucleus Centre in Molecular Ecology and Evolutionary Applications in the Agroecosystems, Universidad de Talca, Talca, Chile. Laboratorio de Ecologia Quimica, Centro Tecnologico de Control Biologico, Instituto de Investigaciones Agropecuarias (INIA)-Quilamapu, Chillan, Chile. Laboratorio de Quimica Ecologica, Departamento de Ciencias Quimicas y Recursos Naturales, Universidad de La Frontera, Temuco, Chile; Centro de Investigacion Biotecnologica Aplicada al Medio Ambiente, CIBAMA, Universidad de La Frontera, Temuco, Chile. Laboratorio de Quimica Ecologica, Departamento de Ciencias Quimicas y Recursos Naturales, Universidad de La Frontera, Temuco, Chile; Centro de Investigacion Biotecnologica Aplicada al Medio Ambiente, CIBAMA, Universidad de La Frontera, Temuco, Chile. Electronic address: herbert.venthur@ufrontera.cl"
Journal Title:		Comp Biochem Physiol Part D Genomics Proteomics
Year:		2018
Volume:		20180430
Issue:
Page Number:		1 - 12
DOI:		10.1016/j.cbd.2018.04.003
ISSN/ISBN:		1878-0407 (Electronic) 1744-117X (Linking)
Abstract:		"The grapevine moth, Lobesia botrana, is considered a harmful pest for vineyards in Chile as well as in North America and Europe. Currently, monitoring and control methods of L. botrana are based on its main sex pheromone component, being effective for low population densities. In order to improve control methods, antennal olfactory proteins in moths, such as odorant-binding proteins (OBPs) and odorant receptors (ORs) have been studied as promising targets for the discovery of new potent semiochemicals, which have not been reported for L. botrana. Therefore, the objective of this study was to identify the repertoire of proteins related to chemoreception in L. botrana by antennal transcriptome and analyze the relative expression of OBPs and CSPs in male and female antennae. Through next-generation sequencing of the antennal transcriptome by Ilumina HiSeq2500 we identified a total of 118 chemoreceptors, from which 61, 42 and 15 transcripts are related to ORs, ionotropic receptors (IRs) and gustatory receptors (GRs), respectively. Furthermore, RNA-Seq data revealed 35 transcripts for OBPs and 18 for chemosensory proteins (CSPs). Analysis by qRT-PCR showed 20 OBPs significantly expressed in female antennae, while 5 were more expressed in males. Similarly, most of the CSPs were significantly expressed in female than male antennae. All the olfactory-related sequences were compared with homologs and their phylogenetic relationships elucidated. Finally, our findings in relation to the improvement of L. botrana management are discussed"
Keywords:		"Animals Arthropod Antennae/metabolism Female Gene Expression Profiling Insect Proteins/*genetics Male Moths/*genetics Phylogeny Receptors, Odorant/*genetics *Transcriptome Vitis/parasitology Antennae Chemosensory receptors Lobesia botrana Relative express;"
Notes:		"MedlineRojas, Valentina Jimenez, Hector Palma-Millanao, Ruben Gonzalez-Gonzalez, Angelica Machuca, Juan Godoy, Ricardo Ceballos, Ricardo Mutis, Ana Venthur, Herbert eng Research Support, Non-U.S. Gov't Netherlands 2018/05/05 Comp Biochem Physiol Part D Genomics Proteomics. 2018 Sep; 27:1-12. doi: 10.1016/j.cbd.2018.04.003. Epub 2018 Apr 30"