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Plant Physiol Biochem

Title:		"MsCYS1, a developmentally-regulated cystatin from alfalfa"
Author(s):		Rivard D; Girard C; Anguenot R; Vezina LP; Trepanier S; Michaud D;
Address:		"CRH/INAF, Pavillon des Services (INAF), Universite Laval, Quebec, Quebec G1K 7P4, Canada"
Journal Title:		Plant Physiol Biochem
Year:		2007
Volume:		20070328
Issue:		6-Jul
Page Number:		508 - 514
DOI:		10.1016/j.plaphy.2007.03.028
ISSN/ISBN:		0981-9428 (Print) 0981-9428 (Linking)
Abstract:		"Several roles have been attributed to cystatins in plants, ranging from the regulation of host [endogenous] cysteine proteases to the inhibition of herbivorous pest [exogenous] proteases. We report here the cloning, expression and functional characterization of a novel cystatin from alfalfa, Medicago sativa L. The new sequence, isolated from a cDNA expression library prepared from young leaves, encodes a protein, MsCYS1, with the typical inhibitory motifs of cystatins, namely the central signature motif QxVxG, a GG doublet in the N-terminal trunk, and a W residue in the C-terminal region, about 30 amino acids distant from the central inhibitory motif. As shown by a protein-based phylogenetic reconstruction, MsCYS1 is a close relative of other cystatins from Fabaceae presumably involved in the regulation of endogenous proteases. This cystatin is developmentally regulated in stems and leaves, and not induced by stress signals including methyl jasmonate, known to activate cystatins involved in plant defense. A recombinant form of MsCYS1 expressed in Escherichia coli was shown to strongly inhibit alfalfa leaf cysteine proteases while showing weak affinity for the digestive cysteine proteases of different herbivorous pests. Overall, these observations suggest an endogenous protease regulatory role for MsCYS1, possibly associated with the early development of stems and leaves"
Keywords:		"Amino Acid Sequence Base Sequence Cloning, Molecular Cystatins/chemistry/*genetics DNA, Complementary/genetics Gene Expression Regulation, Developmental *Gene Expression Regulation, Plant Medicago/genetics Medicago sativa/classification/*genetics Models, ;"
Notes:		"MedlineRivard, Daniel Girard, Cecile Anguenot, Raphael Vezina, Louis-P Trepanier, Sonia Michaud, Dominique eng Research Support, Non-U.S. Gov't France 2007/05/18 Plant Physiol Biochem. 2007 Jun-Jul; 45(6-7):508-14. doi: 10.1016/j.plaphy.2007.03.028. Epub 2007 Mar 28"