| Title: | Identification of the DNA-binding sites for two response regulators involved in control of bacteriocin synthesis in Lactobacillus plantarum C11 |
| Author(s): | Risoen PA; Havarstein LS; Diep DB; Nes IF; |
| Address: | "Department of Biotechnological Sciences, Agricultural University of Norway, As" |
| ISSN/ISBN: | 0026-8925 (Print) 0026-8925 (Linking) |
| Abstract: | "In Lactobacillus plantarum C11, bacteriocin production has previously been shown to be an inducible process, in which a secreted peptide, produced by the host itself, is involved. The inducing factor, designated plantaricin A (PlnA), is a bacteriocin-like peptide encoded by a gene (plnA) located on the same operon as the genes for a two-component regulatory system (plnBCD). This system consists of a histidine kinase (PlnB) and two response regulators (PlnC,D), and belongs to a recently defined subfamily of two-component regulatory systems, which are activated by secreted peptide pheromones through a quorum-sensing mechanism. We show here that the two response regulators PlnC and PlnD bind specifically to imperfect direct repeats found within the adjacent promoter of the plnABCD operon, and to similar sequences found within the promoter regions of two nearby operons containing bacteriocin structural genes (plnEFI and plnJKLR). Binding of PlnC and PlnD was increased two to three fold in the presence of acetyl phosphate. The results suggest that bacteriocin synthesis in L. plantarum C11 is regulated by the DNA-binding activity of the two response regulators PlnC and PlnD" |
| Keywords: | "Bacteriocins/*biosynthesis Base Sequence Binding Sites DNA, Bacterial/chemistry/*metabolism DNA-Binding Proteins/*metabolism Lactobacillus/*genetics/metabolism Molecular Sequence Data Operon/genetics Phosphorylation Promoter Regions, Genetic/*genetics Pro;" |
| Notes: | "MedlineRisoen, P A Havarstein, L S Diep, D B Nes, I F eng Research Support, Non-U.S. Gov't Germany 1998/09/25 Mol Gen Genet. 1998 Aug; 259(2):224-32. doi: 10.1007/pl00008627" |
