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J Ind Microbiol Biotechnol

Title:		Bioluminescent bioreporter integrated-circuit sensing of microbial volatile organic compounds
Author(s):		Ripp S; Daumer KA; McKnight T; Levine LH; Garland JL; Simpson ML; Sayler GS;
Address:		"Center for Environmental Biotechnology, University of Tennessee, 676 Dabney Hall, Knoxville, TN 37996-1605, USA"
Journal Title:		J Ind Microbiol Biotechnol
Year:		2003
Volume:		20031028
Issue:		11
Page Number:		636 - 642
DOI:		10.1007/s10295-003-0093-6
ISSN/ISBN:		1367-5435 (Print) 1367-5435 (Linking)
Abstract:		"A bioluminescent bioreporter for the detection of the microbial volatile organic compound p-cymene was constructed as a model sensor for the detection of metabolic by-products indicative of microbial growth. The bioreporter, designated Pseudomonas putida UT93, contains a Vibrio fischeri luxCDABE gene fused to a p-cymene/p-cumate-inducible promoter derived from the P. putida F1 cym operon. Exposure of strain UT93 to 0.02-850 ppm p-cymene produced self-generated bioluminescence in less than 1.5 h. Signals in response to specific volatile organic compounds (VOCs) such as m- and p-xylene and styrene, also occurred, but at two-fold lower bioluminescent levels. The bioreporter was interfaced with an integrated-circuit microluminometer to create a miniaturized hybrid sensor for remote monitoring of p-cymene signatures. This bioluminescent bioreporter integrated-circuit device was capable of detecting fungal presence within approximately 3.5 h of initial exposure to a culture of p-cymene-producing Penicillium roqueforti"
Keywords:		"Air Microbiology Biotechnology/*methods Cymenes Environmental Monitoring/methods Genes, Reporter Luminescent Proteins/*genetics Monoterpenes/*metabolism Penicillium/growth & development/metabolism Pseudomonas putida/*genetics/metabolism Sick Building Synd;"
Notes:		"MedlineRipp, S Daumer, K A McKnight, T Levine, L H Garland, J L Simpson, M L Sayler, G S eng Research Support, U.S. Gov't, Non-P.H.S. Germany 2003/10/31 J Ind Microbiol Biotechnol. 2003 Nov; 30(11):636-42. doi: 10.1007/s10295-003-0093-6. Epub 2003 Oct 28"