| Title: | "Effects of a small, volatile bacterial molecule on Pseudomonas aeruginosa bacteria using whole cell high-resolution magic angle spinning nuclear magnetic resonance spectroscopy and genomics" |
| Author(s): | Righi V; Constantinou C; Kesarwani M; Rahme LG; Tzika AA; |
| Address: | "NMR Surgical Laboratory, Center for Surgery, Innovation and Bioengineering, Department of Surgery, Massachusetts General and Shriners Burns Hospitals, Harvard Medical School, Boston, MA 02114, USA. Molecular Surgery Laboratory, Department of Surgery, Microbiology and Immunobiology, Harvard Medical School and Molecular Surgery Laboratory, Center for Surgery, Innovation and Bioengineering, Department of Surgery, Massachusetts General and Shriners Burns Hospitals, Boston, MA 02114, USA" |
| ISSN/ISBN: | 1791-244X (Electronic) 1107-3756 (Print) 1107-3756 (Linking) |
| Abstract: | "In the present study, high-resolution magic-angle spinning (HRMAS) nuclear magnetic resonance (NMR) spectroscopy was applied to live Pseudomonas aeruginosa (PA) bacterial cells to determine the metabolome of this opportunistic Gram-negative human pathogen, and in particular, its response to the volatile aromatic low molecular weight signaling molecule, 2-aminoacetophenone (2-AA). Multi-dimensional HRMAS NMR is a promising method which may be used to determine the in vivo metabolome of live intact bacterial cells; 2-AA is produced by PA and triggers the emergence of phenotypes that promote chronic infection phenotypes in in vitro and in vivo (animal) models. In the present study, we applied one-dimensional and two-dimensional proton (1H) HRMAS NMR to PA cells which were grown with or without 2-AA in order to examine the associations between metabolites and cellular processes in response to 2-AA. We also compared whole-genome transcriptome profiles of PA cells grown with or without 2-AA and found that 2-AA promoted profound metabolic changes in the PA cells. By comparing the whole-genome transcriptome profiles and metabolomic analysis, we demonstrated that 2-AA profoundly reprogramed the gene expression and metabolic profiles of the cells. Our in vivo 1H HRMAS NMR spectroscopy may prove to be a helpful tool in the validation of gene functions, the study of pathogenic mechanisms, the classification of microbial strains into functional/clinical groups and the testing of anti-bacterial agents" |
| Keywords: | *Magnetic Resonance Spectroscopy Pseudomonas aeruginosa/cytology/*metabolism Volatile Organic Compounds/analysis/*metabolism; |
| Notes: | "MedlineRighi, Valeria Constantinou, Caterina Kesarwani, Meenu Rahme, Laurence G Tzika, A Aria eng R01 AI134857/AI/NIAID NIH HHS/ R21 AI105902/AI/NIAID NIH HHS/ R33 AI105902/AI/NIAID NIH HHS/ Greece 2018/07/18 Int J Mol Med. 2018 Oct; 42(4):2129-2136. doi: 10.3892/ijmm.2018.3760. Epub 2018 Jul 6" |
