| Title: | Blueberries infected with the fungal pathogen Colletotrichum fioriniae release odors that repel Drosophila suzukii |
| Author(s): | Rering CC; Quadrel A; Urbaneja-Bernat P; Beck JJ; Ben-Zvi Y; Khodadadi F; Acimovic SG; Rodriguez-Saona C; |
| Address: | "Chemistry Research Unit, Center for Medical, Agricultural, and Veterinary Entomology, Agricultural Research Service, United States Department of Agriculture, Gainesville, FL, USA. Department of Entomology, Philip E. Marucci Center, Rutgers University, Chatsworth, NJ, USA. Institut de Recerca i Tecnologia Agroalimentaries (IRTA), Sustainable Plant Protection, Cabrils, Spain. Department of Plant Pathology and Microbiology, University of California, Riverside, CA, USA. Virginia Tech, School of Plant and Environmental Sciences, Alson H. Smith Jr. Agricultural Research and Extension Center, Winchester, VA, USA" |
| ISSN/ISBN: | 1526-4998 (Electronic) 1526-498X (Linking) |
| Abstract: | "BACKGROUND: Spotted-wing drosophila, Drosophila suzukii, is a serious pest of thin-skinned fruits. Alternative methods to control this pest are needed to reduce insecticide use, including new repellents. Previous research demonstrated that D. suzukii adults use odor cues to avoid blueberries infected with the fungal pathogen Colletotrichum fioriniae, which causes the disease anthracnose. To identify novel D. suzukii repellents, we investigated the volatile emission from experimentally-infected fruit, which were inoculated with C. fioriniae isolates in the laboratory, and from field-collected fruit, which were naturally infected and harvested from a field. We then tested the pathogen-induced volatiles on D. suzukii adult behavior. RESULTS: Volatile emission was similar between all five C. fioriniae strains, with good agreement between experimentally-infected and field-collected berries. In total, 14 volatiles were found to be more abundant in infected versus uninfected fruit headspace. In multiple-choice bioassays, nine of the 14 volatiles elicited repellency responses from adult D. suzukii. These nine volatiles were further evaluated in dual choice assays, where all nine reduced fly capture by 43-96% compared to the control. The most repellent compounds tested were the esters ethyl butanoate and ethyl (E)-but-2-enoate, which were more or equally repellent to the known D. suzukii repellents 1-octen-3-ol, geosmin, and 2-pentylfuran. Dose-response assays identified concentration-dependent effects on D. suzukii repellency and oviposition when applied individually and consistent aversion observed across doses of a 1:1 blend. CONCLUSION: We report two repellents from C. fioriniae-infected blueberries that could be useful semiochemicals for the behavioral manipulation of D. suzukii in the field. (c) 2023 Society of Chemical Industry. This article has been contributed to by U.S. Government employees and their work is in the public domain in the USA" |
| Keywords: | Vaccinium spp.anthracnose semiochemical spotted-wing drosophila volatiles; |
| Notes: | "PublisherRering, Caitlin C Quadrel, Amanda Urbaneja-Bernat, Pablo Beck, John J Ben-Zvi, Yahel Khodadadi, Fatemeh Acimovic, Srdan G Rodriguez-Saona, Cesar eng USDA ARS CRIS 6036-224300-001-000D/ USDA SCRI 2020-51181-32140/ Sridhar Polavarapu Memorial Endowment for Vaccinium Research/ LNE22-455R/USDA Sustainable Agriculture Research and Education/ England 2023/08/07 Pest Manag Sci. 2023 Aug 6. doi: 10.1002/ps.7692" |
