| Title: | Comprehensive volatile metabolic fingerprinting of bacterial and fungal pathogen groups |
| Author(s): | Rees CA; Burklund A; Stefanuto PH; Schwartzman JD; Hill JE; |
| Address: | "Geisel School of Medicine at Dartmouth, Hanover, NH 03755, United States of America" |
| ISSN/ISBN: | 1752-7163 (Electronic) 1752-7155 (Print) 1752-7155 (Linking) |
| Abstract: | "The identification of pathogen-specific volatile metabolic 'fingerprints' could lead to the rapid identification of disease-causing organisms either directly from ex vivo patient bio-specimens or from in vitro cultures. In the present study, we have evaluated the volatile metabolites produced by 100 clinical isolates belonging to ten distinct pathogen groups that, in aggregate, account for 90% of bloodstream infections, 90% of urinary tract infections, and 80% of infections encountered in the intensive care unit setting. Headspace volatile metabolites produced in vitro were concentrated using headspace solid-phase microextraction and analyzed via two-dimensional gas chromatography time-of-flight mass spectrometry (HS-SPME-GCxGC-TOFMS). A total of 811 volatile metabolites were detected across all samples, of which 203 were: (1) detected in 9 or 10 (of 10) isolates belonging to one or more pathogen groups, and (2) significantly more abundant in cultures relative to sterile media. Network analysis revealed a distinct metabolic fingerprint associated with each pathogen group, and analysis via Random Forest using leave-one-out cross-validation resulted in a 95% accuracy for the differentiation between groups. The present findings support the results of prior studies that have reported on the differential production of volatile metabolites across pathogenic bacteria and fungi, and provide additional insight through the inclusion of pathogen groups that have seldom been studied previously, including Acinetobacter spp., coagulase-negative Staphylococcus, and Proteus mirabilis, as well as the utilization of HS-SPME-GCxGC-TOFMS for improved sensitivity and resolution relative to traditional gas chromatography-based techniques" |
| Keywords: | Bacteria/*metabolism Fungi/*metabolism Gas Chromatography-Mass Spectrometry Humans Metabolome Metabolomics/*methods Principal Component Analysis Volatile Organic Compounds/*analysis; |
| Notes: | "MedlineRees, Christiaan A Burklund, Alison Stefanuto, Pierre-Hugues Schwartzman, Joseph D Hill, Jane E eng R21 AI121076/AI/NIAID NIH HHS/ T32 LM012204/LM/NLM NIH HHS/ Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't England 2017/09/28 J Breath Res. 2018 Jan 3; 12(2):026001. doi: 10.1088/1752-7163/aa8f7f" |
