| Title: | Non-Invasive Staging of In Vitro Mice Embryos by Means of Volatolomics |
| Author(s): | Raya YSA; Hershkovitz-Pollak Y; Ionescu R; Haick H; |
| Address: | "Department of Chemical Engineering and the Russell Berrie Nanotechnology Institute, Technion - Israel Institute of Technology, Haifa 3200003, Israel. Institute of Veterinary Medicine and Animal Sciences, Estonian University of Life Sciences, 51006 Tartu, Estonia" |
| DOI: | 10.1021/acssensors.2c00792 |
| ISSN/ISBN: | 2379-3694 (Electronic) 2379-3694 (Linking) |
| Abstract: | "Current methods for embryo selection are limited. This study assessed a novel method for the prediction of embryo developmental potential based on the analysis of volatile organic compounds (VOCs) emitted by embryo samples. The study included mice embryos monitored during the pre-implantation period. Four developmental stages of the embryos were tested, covering the period from 1 to 4 days after fecundation. In each stage, the VOCs released by the embryos were collected and examined by employing two different volatolomic techniques, gas-chromatography coupled to mass-spectrometry (GC-MS) and a nanoarray of chemical gas sensors. The GC-MS study revealed that the VOC patterns emanating from embryo samples had statistically different values at different stages of embryo development. The sensor nanoarray was capable of classifying the developmental stages of the embryos. The proposed volatolomics analysis approach for embryos presents a promising potential for predicting their developmental stage. In combination with conventional morphokinetic parameters, it could be effective as a predictive model for detecting metabolic shifts that affect embryo quality before preimplantation" |
| Keywords: | Animals *Embryo Implantation Embryonic Development Gas Chromatography-Mass Spectrometry/methods Mice *Volatile Organic Compounds/analysis Ivf embryo sensor staging volatile organic compound; |
| Notes: | "MedlineRaya, Yasmin Shibli Abu Hershkovitz-Pollak, Yael Ionescu, Radu Haick, Hossam eng Research Support, Non-U.S. Gov't 2022/06/17 ACS Sens. 2022 Jul 22; 7(7):2006-2011. doi: 10.1021/acssensors.2c00792. Epub 2022 Jun 16" |
