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Sci Rep

Title:		An indirect assay for volatile compound production in yeast strains
Author(s):		Ravasio D; Walther A; Trost K; Vrhovsek U; Wendland J;
Address:		"Carlsberg Laboratory; Yeast Genetics Gamle Carlsberg Vej 10 DK-1799 Copenhagen V, Denmark. Fondazione Edmund Mach Research and Innovation Centre Food Quality and Nutrition Department Via E.Mach 1, I-38010 S.Michele all'Adige, Italy"
Journal Title:		Sci Rep
Year:		2014
Volume:		20140115
Issue:
Page Number:		3707 -
DOI:		10.1038/srep03707
ISSN/ISBN:		2045-2322 (Electronic) 2045-2322 (Linking)
Abstract:		"Traditional flavor analysis relies on gas chromatography coupled to mass spectrometry (GC-MS) methods. Here we describe an indirect method coupling volatile compound formation to an ARO9-promoter-LacZ reporter gene. The resulting beta-galactosidase activity correlated well with headspace solid phase micro extraction (HS/SPME) GC-MS data, particularly with respect to the formation of rose flavor. This tool enables large-scale screening of yeast strains and their progeny to identify the most flavor active strains"
Keywords:		Biological Assay/*methods Gas Chromatography-Mass Spectrometry Saccharomyces cerevisiae/*chemistry/*metabolism Solid Phase Extraction Taste/physiology Volatile Organic Compounds/*chemistry beta-Galactosidase/chemistry;
Notes:		"MedlineRavasio, Davide Walther, Andrea Trost, Kajetan Vrhovsek, Urska Wendland, Jurgen eng Research Support, Non-U.S. Gov't England 2014/01/16 Sci Rep. 2014 Jan 15; 4:3707. doi: 10.1038/srep03707"