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Fungal Biol

Title:		The central role of septa in the basidiomycete Schizophyllum commune hyphal morphogenesis
Author(s):		Raudaskoski M;
Address:		"Molecular Plant Biology, Department of Biochemistry, University of Turku, FI-20014 Turku, Finland. Electronic address: marrau@utu.fi"
Journal Title:		Fungal Biol
Year:		2019
Volume:		20190522
Issue:		9
Page Number:		638 - 649
DOI:		10.1016/j.funbio.2019.05.009
ISSN/ISBN:		1878-6146 (Print)
Abstract:		"The purpose of the present research was to observe in the filamentous basidiomycete Schizophyllum commune, the connection between the nuclear division and polymerization of the contractile actin ring with subsequent formation of septa in living hyphae. The filamentous actin was visualized using Lifeact-mCherry and the nuclei with EGFP tagged histone 2B (H2B). Time-lapse fluorescence microscopy confirmed that in monokaryotic and dikaryotic hyphae, the first signs of the contractile actin ring occur at the site of the nuclear division, in one to two minutes after division. At this stage, the telophase nuclei have moved tens of micrometers from the division site. The actin ring is replaced by the septum in six minutes. The apical cells treated with filamentous actin disrupting drug latrunculin A, had swollen tips but the cells were longer than in control samples due to the absence of the actin rings. The nuclear pairing and association with clamp cell development as well as the clamp cell fusion with the subapical cell was disrupted in latrunculin-treated dikaryotic hyphae, indicating that actin filaments are involved in these processes, also regulated by the A and B mating-type genes. This suggests that the actin cytoskeleton may indirectly be a target for mating-type genes"
Keywords:		"Actins/genetics/metabolism Bridged Bicyclo Compounds, Heterocyclic/pharmacology Cell Division/drug effects Cell Nucleus/genetics/metabolism Fungal Proteins/genetics/metabolism Genes, Mating Type, Fungal Hyphae/*cytology/drug effects/genetics/metabolism Mi;"
Notes:		"MedlineRaudaskoski, Marjatta eng Research Support, Non-U.S. Gov't Netherlands 2019/08/17 Fungal Biol. 2019 Sep; 123(9):638-649. doi: 10.1016/j.funbio.2019.05.009. Epub 2019 May 22"