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Int J Food Microbiol

Title:		Bacterial community of industrial raw sausage packaged in modified atmosphere throughout the shelf life
Author(s):		Raimondi S; Nappi MR; Sirangelo TM; Leonardi A; Amaretti A; Ulrici A; Magnani R; Montanari C; Tabanelli G; Gardini F; Rossi M;
Address:		"Department of Life Sciences, University of Modena and Reggio Emilia, Modena, Italy. Department of Life Sciences, University of Modena and Reggio Emilia, Modena, Italy; BIOGEST - SITEIA, University of Modena and Reggio Emilia, Modena, Italy. CLAI S.c.a., Imola, Italy. Centro Interdipartimentale di Ricerca Industriale Agroalimentare, Universita degli Studi di Bologna, Cesena, FC, Italy. Department of Life Sciences, University of Modena and Reggio Emilia, Modena, Italy; BIOGEST - SITEIA, University of Modena and Reggio Emilia, Modena, Italy. Electronic address: maddalena.rossi@unimore.it"
Journal Title:		Int J Food Microbiol
Year:		2018
Volume:		20180428
Issue:
Page Number:		78 - 86
DOI:		10.1016/j.ijfoodmicro.2018.04.041
ISSN/ISBN:		1879-3460 (Electronic) 0168-1605 (Linking)
Abstract:		"Ten lots of industrial raw sausages in modified atmosphere (CO(2) 30%, O(2) 70%), produced in the same plant over 7?ª+months, were analyzed at the day after production (S samples) and at the end of shelf life (E samples), after 12?ª+days storage at 7?ª+ degrees C to simulate thermal abuse. Quality of the products was generally compromised by storage at 7?ª+ degrees C, with only 3 E samples without alterations. During the shelf life, the pH decreased for the accumulation of acetic and lactic acids. A few biogenic amines accumulated, remaining below acceptable limits. The profile of volatile compounds got enriched with alcohols, ketones, and acids (e.g. ethanol, 2,3-butanediol, 2,3-butandione, butanoic acid) originated by bacterial metabolism. Throughout the shelf life, aerobic bacteria increased from 4.7 log to 6.6?ª+log?ª+cfu/g, and lactic acid bacteria (LAB) from 3.7 to 8.1?ª+log?ª+cfu/g. Staphylococci, enterobacteria, and pseudomonads passed from 3.7, 3.0, and 1.7 to 5.5, 4.8, and 3.0?ª+log?ª+cfu/g, respectively. Dominant cultivable LAB, genotyped by RAPD-PCR, belonged to the species Lactobacillus curvatus/graminis and Lactobacillus sakei, with lower amounts of Leuconostoc carnosum and Leuconostoc mesenteroides. Brochothrix thermosphacta was the prevailing species among aerobic bacteria. The same biotypes ascribed to several different species where often found in E samples of diverse batches, suggesting a recurrent contamination from the plant of production. Profiling of 16S rRNA gene evidenced that microbiota of S samples clustered in two main groups where either Firmicutes or Bacteroidetes prevailed, albeit with taxa generally associated to the gastro-intestinal tract of mammals. The microbial diversity was lower in E samples than in S ones. Even though a common profile could not be identified, most E samples clustered together and were dominated by Firmicutes, with Lactobacillaceae and Listeriaceae as the most abundant families (mostly ascribed to Lactobacillus and Brochothrix, respectively). In a sole E sample Proteobacteria (especially Serratia) was the major phylum"
Keywords:		"Acetic Acid/metabolism Animals Bacteria/*classification/genetics/*isolation & purification Colony Count, Microbial Food Microbiology Food Packaging/*methods *Food Preservation *Food Storage Lactic Acid/metabolism Meat Products/*microbiology Microbiota/*ge;"
Notes:		"MedlineRaimondi, Stefano Nappi, Maria Rosaria Sirangelo, Tiziana Maria Leonardi, Alan Amaretti, Alberto Ulrici, Alessandro Magnani, Rudy Montanari, Chiara Tabanelli, Giulia Gardini, Fausto Rossi, Maddalena eng Netherlands 2018/05/22 Int J Food Microbiol. 2018 Sep 2; 280:78-86. doi: 10.1016/j.ijfoodmicro.2018.04.041. Epub 2018 Apr 28"