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Methods Mol Biol

Title:		Metabolomics of plant volatiles
Author(s):		Qualley AV; Dudareva N;
Address:		"Department of Horticulture and Landscape Architecture, Purdue University, West Lafayette, IN, USA"
Journal Title:		Methods Mol Biol
Year:		2009
Volume:		553
Issue:
Page Number:		329 - 343
DOI:		10.1007/978-1-60327-563-7_17
ISSN/ISBN:		1940-6029 (Electronic) 1064-3745 (Linking)
Abstract:		"Plants communicate with their surrounding ecosystems using a diverse array of volatile metabolites that are indicative of the physiological status of the emitter. A variety of systems have been adapted to capture, analyze, identify, and quantify airborne metabolites released by plants. Metabolomic experiments typically involve four steps: sample collection, preparation, product separation, and data analysis. To date, two different types of headspace sampling, static and dynamic, are widely used for volatile metabolome investigation. For static headspace analysis, solid-phase microextraction (SPME) is used to sample volatiles while push and pull as well as closed-loop stripping methods are used for dynamic headspace sampling. After collection, volatile blends are most efficiently and routinely separated prior to analysis using gas chromatography (GC). Sample preparation is simplified because derivatization is not needed with volatile metabolites. GC coupled to detection by electron impact mass spectrometry (EI-MS) provides high chromatographic resolution, sensitivity, compound-specific detection, quantitation, and the potential to identify unknowns by characteristic and reproducible fragmentation spectra in addition to retention time. A variety of resources can be used to identify unknown compounds in a given volatile sample including >600,000 compounds with known mass spectra catalogued in searchable mass spectral libraries"
Keywords:		"Chromatography, Gas/methods Flowers/chemistry/metabolism Metabolomics/*methods Petunia/chemistry/metabolism Plant Extracts/*analysis/metabolism Solid Phase Microextraction/methods Tandem Mass Spectrometry/methods Volatile Organic Compounds/*analysis/metab;"
Notes:		"MedlineQualley, Anthony V Dudareva, Natalia eng Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Review 2009/07/10 Methods Mol Biol. 2009; 553:329-43. doi: 10.1007/978-1-60327-563-7_17"