| Title: | Identification and expression analysis of odorant binding proteins and chemosensory proteins from dissected antennae and mouthparts of the rice bug Leptocorisa acuta |
| Author(s): | Qu MQ; Cui Y; Zou Y; Wu ZZ; Lin JT; |
| Address: | "Guang Zhou City Key Laboratory of Subtropical Fruit Tree Outbreak Control, Zhongkai University of Agriculture and Engineering, Guangzhou 510225, People's Republic of China. Guang Zhou City Key Laboratory of Subtropical Fruit Tree Outbreak Control, Zhongkai University of Agriculture and Engineering, Guangzhou 510225, People's Republic of China. Electronic address: zhongzhen_wu@163.com" |
| Journal Title: | Comp Biochem Physiol Part D Genomics Proteomics |
| DOI: | 10.1016/j.cbd.2019.100631 |
| ISSN/ISBN: | 1878-0407 (Electronic) 1744-117X (Linking) |
| Abstract: | "The rice bug, Leptocorisa acuta (Tunberg) (Hemiptera: Alydidae), is a notorious pest in Asia, and it is significantly attracted by the volatiles derived from host plants. However, it remains unknown how L. acuta recognizes host volatile compounds at the molecular level. Odorant-binding proteins (OBPs) and chemosensory proteins (CSPs) are thought to be responsible for the initial biochemical recognition during olfactory perception. Here, we followed the RNA Sequencing (RNA-Seq) approach to identify candidate genes encoding OBPs and CSPs from dissected antennae and mouthparts of L. acuta. In total, 26 unigenes were identified coding for OBPs (22 Classic OBPs and four Plus-C OBPs), and 17 unigenes coding for CSPs. Real-time quantitative PCR (RT-qPCR) revealed that 11 OBPs (LacuOBP1, 5, 6, 7, 9, 11, 13, 14, 17, 20 and 23) and nine CSPs (LacuCSP2, 3, 4, 5, 6, 8, 9, 10 and 12) were predominantly expressed in antennae, indicating that they might be essential for detection of general odorants and pheromones. Among these antennae-predominantly expressed genes, LacuOBP11 and LacuOBP13 showed male-biased expression and therefore may play crucial roles in the detection of sex pheromones. Seven LacuOBPs (LacuOBP4, 8, 10, 12, 21, 25 and 26) and two CSPs (LacuCSP7 and LacuOBP11) were predominantly expressed in mouthparts, suggesting that these genes might be involved in taste perception. Our work provides a starting point to facilitate functional study of these OBPs and CSPs in L. acuta at the molecular level in the future" |
| Keywords: | "Animals Arthropod Antennae/metabolism Female Hemiptera/*genetics Insect Proteins/*genetics Male Oryza/*parasitology Phylogeny Receptors, Odorant/*genetics *Transcriptome Antenna Chemosensory protein Leptocorisa acuta Mouthparts Odorant binding protein;" |
| Notes: | "MedlineQu, Meng-Qiu Cui, Yang Zou, Yan Wu, Zhong-Zhen Lin, Jin-Tian eng Research Support, Non-U.S. Gov't Netherlands 2019/11/11 Comp Biochem Physiol Part D Genomics Proteomics. 2020 Mar; 33:100631. doi: 10.1016/j.cbd.2019.100631. Epub 2019 Nov 1" |
