| Title: | The antifungal mechanisms of plant volatile compound 1-octanol against Aspergillus flavus growth |
| Author(s): | Qin YL; Zhang SB; Lv YY; Zhai HC; Hu YS; Cai JP; |
| Address: | "School of Biological Engineering, Henan University of Technology, 100 Lianhua Street, Zhengzhou, 450001, People's Republic of China. Henan Provincial Key Laboratory of Biological Processing and Nutritional Function of Wheat, Zhengzhou, 450001, People's Republic of China. School of Biological Engineering, Henan University of Technology, 100 Lianhua Street, Zhengzhou, 450001, People's Republic of China. shbzhang@163.com. Henan Provincial Key Laboratory of Biological Processing and Nutritional Function of Wheat, Zhengzhou, 450001, People's Republic of China. shbzhang@163.com. School of Biological Engineering, Henan University of Technology, 100 Lianhua Street, Zhengzhou, 450001, People's Republic of China. hys308@126.com. Henan Provincial Key Laboratory of Biological Processing and Nutritional Function of Wheat, Zhengzhou, 450001, People's Republic of China. hys308@126.com" |
| Journal Title: | Appl Microbiol Biotechnol |
| DOI: | 10.1007/s00253-022-12049-z |
| ISSN/ISBN: | 1432-0614 (Electronic) 0175-7598 (Linking) |
| Abstract: | "The exploitation of active ingredients from plant volatile organic compounds as natural gaseous fungicides shows remarkable potential for controlling fungal decay in postharvest agroproducts. Although 1-octanol is a common component of cereal volatiles, its antifungal potency against spoilage fungi in postharvest grains remains unclear. In this study, we studied the effectiveness of 1-octanol against Aspergillus flavus growth in postharvest grains and its mechanisms of action. 1-Octanol vapor and liquid contact dose-dependently inhibited A. flavus spore germination and mycelial growth at a low concentration. The simulated storage experiment demonstrated that 300 muL/L of 1-octanol vapor completely controlled A. flavus growth in wheat, corn, and paddy grains with 20% moisture content. 1-Octanol treatment irreversibly damaged the conidial and mycelial morphology of A. flavus and caused electrolyte leakage due to reduced plasma membrane integrity. It induced apoptosis along with morphological abnormalities, phosphatidylserine externalization, mitochondrial membrane potential depolarization, intracellular reactive oxygen species accumulation, and DNA fragmentation in A. flavus cells. Metabolomic analysis revealed that 1-octanol treatment disrupted the biosynthesis of unsaturated fatty acids, ATP-binding cassette transporters, amino acid metabolism, and glycerophospholipid metabolism. This study demonstrated the promising application potential of 1-octanol as a biofumigant for preventing fungal spoilage of postharvest cereal grains. KEY POINTS: * (1) 1-Octanol inhibits Aspergillus flavus growth in the vapor phase and liquid contact; * (2) 1-Octanol damages membrane integrity and induces apoptosis of A. flavus; * (3) Metabolomic changes in A. flavus mycelia were analyzed after 1-octanol treatment" |
| Keywords: | "1-Octanol/metabolism/pharmacology Antifungal Agents/chemistry *Aspergillus flavus *Fungicides, Industrial/pharmacology Spores, Fungal Apoptosis induction Aspergillus flavus Biofumigant Metabolomic analysis Postharvest grain;" |
| Notes: | "MedlineQin, Yu-Liang Zhang, Shuai-Bing Lv, Yang-Yong Zhai, Huan-Chen Hu, Yuan-Sen Cai, Jing-Ping eng Germany 2022/07/03 Appl Microbiol Biotechnol. 2022 Aug; 106(13-16):5179-5196. doi: 10.1007/s00253-022-12049-z. Epub 2022 Jul 2" |
