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J Chromatogr B Analyt Technol Biomed Life Sci

Title:		Determination of volatile biomarkers for apoptosis and necrosis by solid-phase microextraction-gas chromatography/mass spectrometry: a pharmacometabolomic approach to cisplatin's cytotoxicity to human lung cancer cell lines
Author(s):		Pyo JS; Ju HK; Park JH; Kwon SW;
Address:		"Research Institute of Pharmaceutical Sciences and College of Pharmacy, Seoul National University, Seoul 151-742, South Korea"
Journal Title:		J Chromatogr B Analyt Technol Biomed Life Sci
Year:		2008
Volume:		20081101
Issue:		2
Page Number:		170 - 174
DOI:		10.1016/j.jchromb.2008.10.031
ISSN/ISBN:		1570-0232 (Print) 1570-0232 (Linking)
Abstract:		"In order to find potential new biomarkers of cisplatin-induced apoptosis and necrosis, volatile organic compounds (VOCs) from cisplatin-treated human lung cancer cell lines were investigated. The biological system employed was human non-small cell lung carcinoma A549 cell lines. The cell lines were treated with two different concentrations of cisplatin, 100 microM and 400 microM, and apoptosis and necrosis were determined by flow cytometric analysis. For each drug concentration, the VOCs from the treated cell lines were extracted by solid-phase microextraction (SPME), and subsequently analyzed by gas chromatography/mass spectrometry (GC/MS). The compounds that change during cisplatin-induced apoptosis and necrosis of lung cancer cell lines can serve as new biomarkers. The pharmacometabolomic approach presented in this study, significantly, implicates a non-destructive, sample-thrifty and time-saving tool for finding new biomarkers for the assessment of drug-induced cell death pathways"
Keywords:		"Antineoplastic Agents/*toxicity Apoptosis Biomarkers/*analysis Carcinoma, Non-Small-Cell Lung/*drug therapy Cell Line, Tumor Cisplatin/*toxicity Flow Cytometry Gas Chromatography-Mass Spectrometry/economics/*methods Humans Lung Neoplasms/*drug therapy Nec;"
Notes:		"MedlinePyo, Jae Sung Ju, Hyun Kyoung Park, Jeong Hill Kwon, Sung Won eng Evaluation Study Research Support, Non-U.S. Gov't Netherlands 2008/11/26 J Chromatogr B Analyt Technol Biomed Life Sci. 2008 Dec 15; 876(2):170-4. doi: 10.1016/j.jchromb.2008.10.031. Epub 2008 Nov 1"