| Title: | Exploring the potential of needle trap microextraction combined with chromatographic and statistical data to discriminate different types of cancer based on urinary volatomic biosignature |
| Author(s): | Porto-Figueira P; Pereira JAM; Camara JS; |
| Address: | "CQM-UMa, Centro de Quimica da Madeira, Universidade da Madeira, Campus Universitario da Penteada, 9020-105 Funchal, Portugal. CQM-UMa, Centro de Quimica da Madeira, Universidade da Madeira, Campus Universitario da Penteada, 9020-105 Funchal, Portugal; Faculdade das Ciencias Exatas e da Engenharia da Universidade da Madeira, Campus Universitario da Penteada, 9020-105 Funchal, Portugal. Electronic address: jsc@uma.pt" |
| DOI: | 10.1016/j.aca.2018.04.027 |
| ISSN/ISBN: | 1873-4324 (Electronic) 0003-2670 (Linking) |
| Abstract: | "The worldwide high cancer incidence and mortality demands for more effective and specific diagnostic strategies. In this study, we evaluated the efficiency of an innovative methodology, Needle Trap Microextraction (NTME), combined with gas chromatography-mass spectrometry (GC-MS), for the establishment of the urinary volatomic biosignature from breast (BC), and colon (CC) cancer patients as well as healthy individuals (CTL). To achieve this, 40?ª+mL of the headspace of acidified urine (4?ª+mL, 20% NaCl, pH?ª+=?ª+2), equilibrated at 50?ª+ degrees C during 40?ª+min, were loaded through the DVB/Car1000/CarX sorbent inside the NTD, and subjected to a GC-MS analysis. This allowed the identification of 130 VOMs from different chemical families that were further processed using discriminant analysis through the partial least squares method (PLS-DA). Several pathways are over activated in cancer patients, being phenylalanine pathway in BC and limonene and pinene degradation pathway in CC the most relevant. Butanoate metabolism is also highly activated in both cancers, as well as tyrosine metabolism in a lesser extension. In BC the xenobiotics metabolism by cytochrome P450 and fatty acid biosynthesis are also differentially activated. Different clusters corresponding to the groups recruited allowed to define sets of volatile organic metabolites (VOMs fingerprints) that exhibit high classification rates, sensitivity and specificity in the discrimination of the selected cancers. As far as we are aware, this is the first time that NTME is used for isolation urinary volatile metabolites, being the obtained results very promising" |
| Keywords: | Breast Neoplasms/*chemistry Colonic Neoplasms/*chemistry Discriminant Analysis Female Gas Chromatography-Mass Spectrometry Humans Least-Squares Analysis Needles *Solid Phase Microextraction Volatile Organic Compounds/*analysis Biomarkers Cancer Needle tra; |
| Notes: | "MedlinePorto-Figueira, Priscilla Pereira, Jorge A M Camara, Jose S eng Netherlands 2018/05/15 Anal Chim Acta. 2018 Sep 6; 1023:53-63. doi: 10.1016/j.aca.2018.04.027. Epub 2018 Apr 24" |
