| Title: | "DLX-2, MASH-1, and MAP-2 expression and bromodeoxyuridine incorporation define molecularly distinct cell populations in the embryonic mouse forebrain" |
| Author(s): | Porteus MH; Bulfone A; Liu JK; Puelles L; Lo LC; Rubenstein JL; |
| Address: | "Nina Ireland Laboratory of Developmental Biology, Department of Psychiatry, University of California, San Francisco 94143-0984" |
| DOI: | 10.1523/JNEUROSCI.14-11-06370.1994 |
| ISSN/ISBN: | 0270-6474 (Print) 1529-2401 (Electronic) 0270-6474 (Linking) |
| Abstract: | "Recently, the Dlx family of homeobox genes have been identified as candidates for regulating patterning and differentiation of the forebrain. We have made a polyclonal antiserum to the protein product of the Dlx-2 gene. Using this antiserum, we have characterized the spatial and temporal pattern of DLX-2 protein expression during murine development and in the adult mouse brain. These studies demonstrate that, like the mRNA from the Dlx-2 gene, DLX-2 protein is expressed in mouse embryonic forebrain, limbs, tail, genital tubercle, and branchial arches. Within the embryonic forebrain, DLX-2 protein is expressed within specific transverse and longitudinal domains. Analysis of expression within the wall of the forebrain shows that DLX-2 is expressed in proliferative regions including the ventricular and subventricular zones. DLX-2 is expressed in the same cells as MASH-1, a marker of relatively undifferentiated cells, but in a reciprocal fashion to MAP-2, a marker of terminal neuronal differentiation. A number of DLX-2-expressing cells, but not all, can be labeled with bromodeoxyuridine (BrdU). Using the patterns of DLX-2, MASH-1, MAP-2 expression, and bromodeoxyuridine incorporation, we identify four molecularly distinct populations of cells that may correspond to different stages of neuronal differentiation in the mouse basal forebrain, in which DLX-2 is expressed at the transition from proliferation to terminal differentiation" |
| Keywords: | "Animals Basic Helix-Loop-Helix Transcription Factors Bromodeoxyuridine/*metabolism DNA-Binding Proteins/metabolism Embryo, Mammalian/cytology/*physiology Fungal Proteins Ganglia/embryology *Gene Expression *Genes, Homeobox Immunohistochemistry Median Emin;" |
| Notes: | "MedlinePorteus, M H Bulfone, A Liu, J K Puelles, L Lo, L C Rubenstein, J L eng KO2 MH01046-01/MH/NIMH NIH HHS/ R01 MH49428-01/MH/NIMH NIH HHS/ Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S. 1994/11/01 J Neurosci. 1994 Nov; 14(11 Pt 1):6370-83. doi: 10.1523/JNEUROSCI.14-11-06370.1994" |
