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Biochim Biophys Acta

Title:		"Novobiocin activates the mating response in yeast through the alpha-pheromone receptor, Ste2p"
Author(s):		Pocklington MJ; Orr E;
Address:		"Department of Genetics, University of Leicester, UK"
Journal Title:		Biochim Biophys Acta
Year:		1994
Volume:		1224
Issue:		3
Page Number:		401 - 412
DOI:		10.1016/0167-4889(94)90275-5
ISSN/ISBN:		0006-3002 (Print) 0006-3002 (Linking)
Abstract:		"In a previous publication we reported the isolation of several yeast mutants sensitive to the coumarin drug novobiocin. Here we characterise the wild-type gene (NBS5), obtained by rescuing a novobiocin-sensitive nbs5 strain to natural novobiocin resistance. We show that nbs5 is allelic to the previously characterised gene sst2, conferring super-sensitivity to the mating pheromones. Although nbs5/sst2 cells of both mating types are super-sensitive to the opposite mating pheromone, only cells of mating-type a are sensitive to novobiocin. We show that the entire effect of the drug is mediated through Ste2p, the alpha-pheromone receptor. Thus, novobiocin is a functional agonist of Ste2p, and may identify a potentially useful interaction between coumarin drugs and the family of G-protein-coupled receptors"
Keywords:		"Genetic Complementation Test Mating Factor Novobiocin/*pharmacology Peptides/*genetics/physiology Plasmids Receptors, Mating Factor Receptors, Peptide/*agonists/genetics Saccharomyces cerevisiae/*drug effects/genetics/physiology Signal Transduction *Trans;"
Notes:		"MedlinePocklington, M J Orr, E eng Wellcome Trust/United Kingdom Research Support, Non-U.S. Gov't Netherlands 1994/12/30 Biochim Biophys Acta. 1994 Dec 30; 1224(3):401-12. doi: 10.1016/0167-4889(94)90275-5"