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J Investig Allergol Clin Immunol

Title:		Inflammatory Asthma Phenotype Discrimination Using an Electronic Nose Breath Analyzer
Author(s):		Plaza V; Crespo A; Giner J; Merino JL; Ramos-Barbon D; Mateus EF; Torrego A; Cosio BG; Agusti A; Sibila O;
Address:
Journal Title:		J Investig Allergol Clin Immunol
Year:		2015
Volume:		25
Issue:		6
Page Number:		431 - 437
DOI:
ISSN/ISBN:		1018-9068 (Print) 1018-9068 (Linking)
Abstract:		"BACKGROUND AND OBJECTIVE: Patients with persistent asthma have different inflammatory phenotypes. The electronic nose is a new technology capable of distinguishing volatile organic compound (VOC) breath-prints in exhaled breath. The aim of the study was to investigate the capacity of electronic nose breath-print analysis to discriminate between different inflammatory asthma phenotypes (eosinophilic, neutrophilic, paucigranulocytic) determined by induced sputum in patients with persistent asthma. METHODS: Fifty-two patients with persistent asthma were consecutively included in a cross-sectional proof-of-concept study. Inflammatory asthma phenotypes (eosinophilic, neutrophilic and paucigranulocytic) were recognized by inflammatory cell counts in induced sputum. VOC breath-prints were analyzed using the electronic nose Cyranose 320 and assessed by discriminant analysis on principal component reduction, resulting in cross-validated accuracy values. Receiver operating characteristic (ROC) curves were calculated. RESULTS: VOC breath-prints were different in eosinophilic asthmatics compared with both neutrophilic asthmatics (accuracy 73%; P=.008; area under ROC, 0.92) and paucigranulocytic asthmatics (accuracy 74%; P=.004; area under ROC, 0.79). Likewise, neutrophilic and paucigranulocytic breath-prints were also different (accuracy 89%; P=.001; area under ROC, 0.88). CONCLUSION: An electronic nose can discriminate inflammatory phenotypes in patients with persistent asthma in a regular clinical setting. ClinicalTrials.gov identifier: NCT02026336"
Keywords:		Adult Aged Asthma/*immunology Cross-Sectional Studies *Electronic Nose Female Humans Inflammation/*immunology Male Middle Aged Phenotype Volatile Organic Compounds/*analysis;
Notes:		"MedlinePlaza, V Crespo, A Giner, J Merino, J L Ramos-Barbon, D Mateus, E F Torrego, A Cosio, B G Agusti, A Sibila, O eng Research Support, Non-U.S. Gov't Spain 2016/01/29 J Investig Allergol Clin Immunol. 2015; 25(6):431-7"