| Title: | An in vitro model to evaluate the inflammatory response after gaseous formaldehyde exposure of lung epithelial cells |
| Author(s): | Persoz C; Achard S; Leleu C; Momas I; Seta N; |
| Address: | "Universite Paris Descartes, Laboratoire de Sante Publique et Environnement - EA 4064, Paris, France" |
| DOI: | 10.1016/j.toxlet.2010.03.003 |
| ISSN/ISBN: | 1879-3169 (Electronic) 0378-4274 (Linking) |
| Abstract: | "Asthma is a public health problem worldwide, and indoor air pollution considered to be a potential etiology. New tools need to be developed to study the effects of air pollutants in vitro and modelize inhalation exposure. This study was thus set up to design an in vitro model, using a direct exposure device to study the cellular effects of air pollutants at environmental doses on lung epithelial cells, and apply this to gaseous formaldehyde (FA). A549 cells were exposed using the direct exposure device (air/liquid interface) to FA without, after and before TNFalpha (1 ng/mL) sensitization. 24h after exposure, cellular viability (XTT) and inflammation (IL-6, IL-8 and MCP-1) were assessed. No effects on cellular viability were observed for concentrations < or =50 microg/m(3). After TNFalpha sensitization, FA-exposure induced a significant increase in IL-8 (p<0.001), which could lead to the initiation or pathogenesis of non-specific respiratory inflammation. The results of this study demonstrate the feasibility and sensitivity of the exposure system for testing inflammatory cellular effects of indoor gaseous compounds at environmental doses directly on human respiratory cells" |
| Keywords: | Cell Line Cell Survival Chemokine CCL2/metabolism Environmental Exposure/*analysis Epithelial Cells/*drug effects/metabolism Formaldehyde/*toxicity Inflammation Mediators/*metabolism Interleukin-6/metabolism Interleukin-8/metabolism Lung/*drug effects/met; |
| Notes: | "MedlinePersoz, Charles Achard, Sophie Leleu, Christopher Momas, Isabelle Seta, Nathalie eng Research Support, Non-U.S. Gov't Netherlands 2010/03/17 Toxicol Lett. 2010 Jun 2; 195(2-3):99-105. doi: 10.1016/j.toxlet.2010.03.003. Epub 2010 Mar 10" |
