| Title: | "A novel salivary effector, BtE3, is essential for whitefly performance on host plants" |
| Author(s): | Peng Z; Su Q; Ren J; Tian L; Zeng Y; Yang Y; Wang S; Xie W; Wu Q; Li Z; Zhang Y; |
| Address: | "State Key Laboratory of Vegetable Biobreeding, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, China. Institute of Plant Protection, Guangdong Academy of Agricultural Sciences and Guangdong Provincial Key Laboratory of High Technology for Plant Protection, Guangzhou 510640, China. Ministry of Agriculture and Rural Affairs Key Laboratory of Sustainable Crop Production in the Middle Reaches of the Yangtze River (Co-construction by Ministry and Province), Hubei Engineering Technology Center for Pest Forewarning and Management, College of Agriculture, Yangtze University, Jingzhou, Hubei 434025, China. Institute of Plan Protection, Beijing Academy of Agriculture and Forestry Science, Beijing 100097, China. School of Life Sciences, Hubei Normal University, Huangshi 435002, China" |
| ISSN/ISBN: | 1460-2431 (Electronic) 0022-0957 (Linking) |
| Abstract: | "The whitefly Bemisia tabaci is a piercing-sucking herbivore that reduces the yields of crops both by feeding on plants and transmitting plant viruses. Like most plant feeders, B. tabaci has evolved ways to avoid plant defence responses. For example, B. tabaci is known to secrete salivary effectors to suppress host defences. However, the nature of B. tabaci effectors is not completely understood. In this study, we used B. tabaci genomic and salivary gland transcriptomic data and an overexpression system to identify a previously unknown B. tabaci salivary effector, BtE3. BtE3 is specifically expressed in the head (containing primary salivary glands) and is secreted into hosts during B. tabaci feeding. In planta overexpression of BtE3 blocked Burkholderia glumae-induced hypersensitive response (HR) in both Nicotiana benthamiana and Solanum lycopersicum. Silencing of BtE3 by plant-mediated RNAi prevented B. tabaci from continuously ingesting phloem sap, and reduced B. tabaci survival and fecundity. Moreover, overexpression of BtE3 in planta up-regulated the salicylic acid- (SA-) signalling pathway, but suppressed the downstream jasmonic acid- (JA-) mediated defences. Taken together, these results indicate that BtE3 is a B. tabaci-specific novel effector involved in B. tabaci-plant interactions. These findings increase our understanding of B. tabaci effectors and suggest novel strategies for B. tabaci pest management" |
| Keywords: | "Animals *Hemiptera/physiology Tobacco/genetics Signal Transduction *Solanum lycopersicum/genetics Crops, Agricultural Bemisia tabaci Burkholderia glumae transient assay feeding behaviour herbivore-host interaction phytohormones salivary effectors;" |
| Notes: | "MedlinePeng, Zhengke Su, Qi Ren, Jun Tian, Lixia Zeng, Yang Yang, Yuting Wang, Shaoli Xie, Wen Wu, Qingjun Li, Zhenyu Zhang, Youjun eng Research Support, Non-U.S. Gov't England 2023/01/18 J Exp Bot. 2023 Mar 28; 74(6):2146-2159. doi: 10.1093/jxb/erad024" |
