| Title: | Nuclear relocation of Kss1 contributes to the specificity of the mating response |
| Address: | "Department of Fundamental Microbiology University of Lausanne Lausanne, Switzerland" |
| ISSN/ISBN: | 2045-2322 (Electronic) 2045-2322 (Linking) |
| Abstract: | "Mitogen Activated Protein Kinases (MAPK) play a central role in transducing extra-cellular signals into defined biological responses. These enzymes, conserved in all eukaryotes, exert their function via the phosphorylation of numerous substrates located throughout the cell and by inducing a complex transcriptional program. The partitioning of their activity between the cytoplasm and the nucleus is thus central to their function. Budding yeast serves as a powerful system to understand the regulation of these fundamental biological phenomena. Under vegetative growth, the MAPK Kss1 is enriched in the nucleus of the cells. Stimulation with mating pheromone results in a rapid relocation of the protein in the cytoplasm. Activity of either Fus3 or Kss1 in the mating pathway is sufficient to drive this change in location by disassembling the complex formed between Kss1, Ste12 and Dig1. Artificial enrichment of the MAPK Kss1 in the nucleus in presence of mating pheromone alters the transcriptional response of the cells and induces a cell-cycle arrest in absence of Fus3 and Far1" |
| Keywords: | "Alleles Cell Cycle Checkpoints Cell Nucleus/*metabolism Enzyme Activation Fungal Proteins/*metabolism Gene Expression Regulation, Fungal Mitogen-Activated Protein Kinases/genetics/*metabolism Models, Biological Protein Binding Protein Transport Signal Tra;" |
| Notes: | "MedlinePelet, Serge eng Research Support, Non-U.S. Gov't England 2017/03/07 Sci Rep. 2017 Mar 6; 7:43636. doi: 10.1038/srep43636" |
