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Cell Tissue Res

Title:		Gamma-aminobutyric acid immunostaining in the antennal lobe of the moth Heliothis virescens and its colocalization with neuropeptides
Author(s):		Berg BG; Schachtner J; Homberg U;
Address:		"Neuroscience Unit/Department of Psychology, Norwegian University of Science and Technology, 7489 Trondheim, Norway. Bente.Berg@ntnu.no"
Journal Title:		Cell Tissue Res
Year:		2009
Volume:		20090121
Issue:		3
Page Number:		593 - 605
DOI:		10.1007/s00441-008-0744-z
ISSN/ISBN:		1432-0878 (Electronic) 0302-766X (Linking)
Abstract:		"The antennal lobe is the primary processing center for olfactory information in insects. To understand further the neural circuitry of this brain area, we have investigated the distribution of gamma-aminobutyric acid (GABA) and its colocalization with neuropeptides in the antennal lobe of the noctuid moth Heliothis virescens. Immunocytochemical experiments with an antiserum against GABA showed a large number of labeled somata in the antennal lobe; these somata were located exclusively in the lateral cell cluster. Stained neurites innervating all antennal-lobe glomeruli, including the male-specific macroglomerular complex, suggested a prominent role of GABA in processing olfactory information, including signals from pheromones, interspecifically acting odors, and plant odors. Fibers in two antennocerebral tracts (the middle and dorsal antennocerebral tract) exhibited prominent GABA immunoreactivity. Double-labeling experiments revealed that immunostaining for three neuropeptides, viz., A-type allatostatin, Manduca sexta allatotropin, and FMRFamide-related peptides, was largely colocalized with GABA in cell bodies of the lateral cell cluster. The general absence of peptide immunostaining in the antennocerebral tracts strongly indicated that these peptides were colocalized with GABA in local interneurons of the antennal lobe. In contrast, tachykinin-related peptides occurred in a distinct population of local antennal-lobe neurons that did not exhibit GABA immunostaining. Thus, local interneurons that were not GABAergic were present in the moth antennal lobe"
Keywords:		"Animals Immunohistochemistry Male Microscopy, Confocal Moths/*metabolism Neurons/metabolism/ultrastructure Neuropeptides/*metabolism Olfactory Pathways/*metabolism/ultrastructure gamma-Aminobutyric Acid/analysis/*metabolism;neuroscience;"
Notes:		"MedlineBerg, Bente G Schachtner, Joachim Homberg, Uwe eng Research Support, Non-U.S. Gov't Germany 2009/01/22 Cell Tissue Res. 2009 Mar; 335(3):593-605. doi: 10.1007/s00441-008-0744-z. Epub 2009 Jan 21"