| Title: | "Electrophysiological Responses of the Beech Leaf-Mining Weevil, Orchestes fagi, to Seasonally-Variant Volatile Organic Compounds Emitted by American Beech, Fagus grandifolia" |
| Author(s): | Pawlowski SP; Sweeney JD; Hillier NK; |
| Address: | "Department of Biology, Acadia University, Wolfville, Nova Scotia, Canada. simonpawlowski@acadiau.ca. Natural Resources Canada, Canadian Forest Service-Atlantic Forestry Centre, Fredericton, New Brunswick, Canada. Department of Biology, Acadia University, Wolfville, Nova Scotia, Canada" |
| DOI: | 10.1007/s10886-020-01216-z |
| ISSN/ISBN: | 1573-1561 (Electronic) 0098-0331 (Linking) |
| Abstract: | "The beech leaf-mining weevil, Orchestes fagi, is a common pest of European beech, Fagus sylvatica, and has recently become established in Nova Scotia, Canada where it similarly infests American beech, F. grandifolia. We collected volatile organic compounds (VOCs) emitted by F. grandifolia leaves at five developmental stages over one growing season and simultaneously analyzed them for volatile emissions and O. fagi antennal response using gas chromatography-electroantennographic detection (GC-EAD). Volatile profiles changed significantly throughout the growing season, shifting from primarily beta-caryophyllene, methyl jasmonate, and simple monoterpene emissions to dominance of the bicyclic monoterpene sabinene during maturity. Two VOCs dominant during bud burst, (R)-(+)-limonene and geranyl-p-cymene, may be of biological relevance due to the highly specific oviposition period of O. fagi at this stage though antennal responses were inconclusive. Senescence showed a decrease in blend complexity with an increase in (Z)-3-hexenyl acetate and (Z)-3-hexen-1-ol as well as a resurgence of alpha-terpinene and geranyl-p-cymene. We present a novel electroantennal preparation for O. fagi. Antennae of both male and female O. fagi responded to the majority of detectable peaks for host volatiles presented via GC-EAD. Females displayed greater overall sensitivities and less specificity to host volatiles and it is hypothesized that this translates to more generalist olfaction than males. It is clear that olfactory cues are important physiologically though their implications on behaviour are still unknown. The results presented in this study provide a baseline and tools on which to connect the complex and highly time-specific phenology of both F. grandifolia and the destructive pest O. fagi through which olfactory-based lures can be investigated for monitoring systems" |
| Keywords: | "Animals Behavior, Animal/drug effects Electrophysiological Phenomena Fagus/*chemistry/growth & development Female Gas Chromatography-Mass Spectrometry Larva/physiology Male North America Olfactory Perception/drug effects Volatile Organic Compounds/*isolat;" |
| Notes: | "MedlinePawlowski, Simon P Sweeney, Jon D Hillier, N Kirk eng 197853/Atlantic Canada Opportunities Agency Atlantic Innovation Fund/ 22087/Canada Foundation for Innovation/ 356109-2008 RGPIN/Natural Sciences and Engineering Research Council of Canada/ 2020/09/12 J Chem Ecol. 2020 Oct; 46(10):935-946. doi: 10.1007/s10886-020-01216-z. Epub 2020 Sep 11" |
