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J Exp Bot

Title:		Dynamic histone acetylation in floral volatile synthesis and emission in petunia flowers
Author(s):		Patrick RM; Huang XQ; Dudareva N; Li Y;
Address:		"Department of Horticulture and Landscape Architecture, Purdue University, West Lafayette, IN 47907,USA. Purdue Center for Plant Biology, Purdue University, West Lafayette, IN 47907,USA. Department of Biochemistry, Purdue University, West Lafayette, IN 47907,USA"
Journal Title:		J Exp Bot
Year:		2021
Volume:		72
Issue:		10
Page Number:		3704 - 3722
DOI:		10.1093/jxb/erab072
ISSN/ISBN:		1460-2431 (Electronic) 0022-0957 (Print) 0022-0957 (Linking)
Abstract:		"Biosynthesis of secondary metabolites relies on primary metabolic pathways to provide precursors, energy, and cofactors, thus requiring coordinated regulation of primary and secondary metabolic networks. However, to date, it remains largely unknown how this coordination is achieved. Using Petunia hybrida flowers, which emit high levels of phenylpropanoid/benzenoid volatile organic compounds (VOCs), we uncovered genome-wide dynamic deposition of histone H3 lysine 9 acetylation (H3K9ac) during anthesis as an underlying mechanism to coordinate primary and secondary metabolic networks. The observed epigenome reprogramming is accompanied by transcriptional activation at gene loci involved in primary metabolic pathways that provide precursor phenylalanine, as well as secondary metabolic pathways to produce volatile compounds. We also observed transcriptional repression among genes involved in alternative phenylpropanoid branches that compete for metabolic precursors. We show that GNAT family histone acetyltransferase(s) (HATs) are required for the expression of genes involved in VOC biosynthesis and emission, by using chemical inhibitors of HATs, and by knocking down a specific HAT gene, ELP3, through transient RNAi. Together, our study supports that regulatory mechanisms at chromatin level may play an essential role in activating primary and secondary metabolic pathways to regulate VOC synthesis in petunia flowers"
Keywords:		"Acetylation Flowers/genetics/metabolism Gene Expression Regulation, Plant Histones/genetics *Petunia/genetics/metabolism Plant Proteins/genetics/metabolism *Volatile Organic Compounds Epigenome floral volatile histone acetylation organic compounds seconda;"
Notes:		"MedlinePatrick, Ryan M Huang, Xing-Qi Dudareva, Natalia Li, Ying eng Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. England 2021/02/20 J Exp Bot. 2021 May 4; 72(10):3704-3722. doi: 10.1093/jxb/erab072"