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J Chem Ecol

Title:		The Aggregation Pheromone of Phyllotreta striolata (Coleoptera: Chrysomelidae) Revisited
Author(s):		Beran F; Jimenez-Aleman GH; Lin MY; Hsu YC; Mewis I; Srinivasan R; Ulrichs C; Boland W; Hansson BS; Reinecke A;
Address:		"Department of Evolutionary Neuroethology, Max Planck Institute for Chemical Ecology, Hans-Knoell-Strasse 8, D-07745, Jena, Germany. fberan@ice.mpg.de. Department of Bioorganic Chemistry, Max Planck Institute for Chemical Ecology, Hans-Knoell-Strasse 8, D-07745, Jena, Germany. AVRDC-The World Vegetable Center, Entomology Unit, 60 Yi-min Liao, Shanhua, 74151, Tainan, Taiwan. Department Urban Ecophysiology, Humboldt-Universitat zu Berlin, Lentzeallee 55-57, D-14195, Berlin, Germany. Plant Analysis and Stored Product Protection, Institute for Ecological Chemistry, Julius Kuhn-Institut, Konigin-Luise-Str. 19, D-14195, Berlin, Germany. Department of Evolutionary Neuroethology, Max Planck Institute for Chemical Ecology, Hans-Knoell-Strasse 8, D-07745, Jena, Germany. Behavioural Ecology and Evolutionary Genetics, Max Planck Institute for Ornithology, Eberhard-Gwinner-Str. 7, 82319, Seewiesen, Germany"
Journal Title:		J Chem Ecol
Year:		2016
Volume:		20160812
Issue:		8
Page Number:		748 - 755
DOI:		10.1007/s10886-016-0743-6
ISSN/ISBN:		1573-1561 (Electronic) 0098-0331 (Print) 0098-0331 (Linking)
Abstract:		"Aggregations of the striped flea beetle Phyllotreta striolata on their crucifer host plants are mediated by volatiles emitted from feeding males. The male-specific sesquiterpene, (6R,7S)-himachala-9,11-diene (compound A), was shown previously to be physiologically and behaviorally active, but compound A was attractive only when combined with unnaturally high doses of the host plant volatile allyl isothiocyanate (AITC) in field trapping experiments. This indicated that our understanding of the chemical communication in this species is incomplete. Another male-specific sesquiterpenoid, (3S,9R,9aS)-3-hydroxy-3,5,5,9-tetramethyl-5,6,7,8,9,9a-hexahydro-1H-benzo[7]annulen-2(3H)-one (compound G), has been reported from an American P. striolata population. We confirmed the presence of compound G, and investigated its interaction with compound A and AITC in a P. striolata population in Taiwan. Compound G was attractive to Taiwanese P. striolata in laboratory bioassays, but significantly more beetles were attracted to a blend of compounds A and G. Under the same conditions, P. striolata showed no preference for the blend of A and G combined with a range of doses of AITC over the sesquiterpenoid blend alone. The sesquiterpenoid blend was tested further in field trapping experiments and attracted significantly more beetles than traps baited with compound A and ecologically relevant amounts of AITC. We conclude that A and G are components of the male-specific aggregation pheromone of P. striolata in Taiwan, and that the attractiveness of the pheromone is not reliant on the presence of AITC. Our results further indicate that the male-specific sesquiterpenoid blends differ qualitatively between the Taiwanese and American populations of P. striolata"
Keywords:		"Aggression/*drug effects Animals Behavior, Animal/drug effects Biological Assay Coleoptera/*drug effects Drug Interactions Isothiocyanates/pharmacology Male Pheromones/analysis/chemistry/*pharmacology Sesquiterpenes/analysis/chemistry/pharmacology Sex Cha;"
Notes:		"MedlineBeran, Franziska Jimenez-Aleman, Guillermo H Lin, Mei-Ying Hsu, Yun-Che Mewis, Inga Srinivasan, Ramasamy Ulrichs, Christian Boland, Wilhelm Hansson, Bill S Reinecke, Andreas eng 2016/08/16 J Chem Ecol. 2016 Aug; 42(8):748-755. doi: 10.1007/s10886-016-0743-6. Epub 2016 Aug 12"