« Previous AbstractChemical Prey Luring in Jackson's Chameleons    Next AbstractIn Search for Pheromone Receptors: Certain Members of the Odorant Receptor Family in the Desert Locust Schistocerca gregaria (Orthoptera: Acrididae) Are Co-expressed with SNMP1 »

Int J Biol Sci

Title:		Expression of a GABA(B) - receptor in olfactory sensory neurons of sensilla trichodea on the male antenna of the moth Heliothis virescens
Author(s):		Pregitzer P; Schultze A; Raming K; Breer H; Krieger J;
Address:		"University of Hohenheim, Institute of Physiology, Stuttgart, Germany"
Journal Title:		Int J Biol Sci
Year:		2013
Volume:		20130720
Issue:		7
Page Number:		707 - 715
DOI:		10.7150/ijbs.6674
ISSN/ISBN:		1449-2288 (Electronic) 1449-2288 (Linking)
Abstract:		"In the olfactory pathway of Drosophila, a GABAB receptor mediated presynaptic gain control mechanism at the first synapse between olfactory sensory neurons (OSNs) and projection neurons has been suggested to play a critical role in setting the sensitivity and detection range of the sensory system. To approach the question if such a mechanism may be realized in the pheromone recognition system of male moths in this study attempts were made to explore if moth's pheromone-responsive cells express a GABAB- receptor. Employing a combination of genome analysis, RT-PCR experiments and screening of an antennal cDNA library we have identified a cDNA which encodes the GABAB-R1 receptor of Heliothis virescens. Moreover, based on the HvirGABAB-R1 sequence we could predict a GABAB-R1 protein from genome sequences of the silkmoth Bombyx mori. To assess whether HvirGABAB-R1 is expressed in OSNs of male antenna we performed whole-mount in situ hybridization (WM-ISH) experiments. Several HvirGABAB-R1 positive cells were visualized under long sensilla trichodea, known to contain pheromone-responsive OSNs. In parallel it was shown that cells under long trichoid hairs were labelled with pheromone receptor specific probes. In addition, the HvirGABAB-R1 specific probe also labelled several cells under shorter olfactory sensilla, but never stained cells under mechanosensory/gustatory sensilla chaetica. Together, the results indicate that a GABAB receptor is expressed in pheromone-responsive OSNs of H. virescens and suggest a presynaptic gain control mechanism in the axon terminals of these cells"
Keywords:		"Amino Acid Sequence Animals Male Molecular Sequence Data Moths Receptors, GABA-B/*biosynthesis Receptors, Pheromone/*biosynthesis Sensilla/metabolism Sensory Receptor Cells/metabolism Sequence Alignment Gaba in situ hybridization.moth olfaction pheromone;"
Notes:		"MedlinePregitzer, Pablo Schultze, Anna Raming, Klaus Breer, Heinz Krieger, Jurgen eng R01 HG004164/HG/NHGRI NIH HHS/ Research Support, Non-U.S. Gov't Australia 2013/08/02 Int J Biol Sci. 2013 Jul 20; 9(7):707-15. doi: 10.7150/ijbs.6674. Print 2013"