| Title: | "Genomic analysis of three sponge-associated Arthrobacter Antarctic strains, inhibiting the growth of Burkholderia cepacia complex bacteria by synthesizing volatile organic compounds" |
| Author(s): | Orlandini V; Maida I; Fondi M; Perrin E; Papaleo MC; Bosi E; de Pascale D; Tutino ML; Michaud L; Lo Giudice A; Fani R; |
| Address: | "Laboratory of Microbial and Molecular Evolution, Department of Biology, University of Florence, Via Madonna del Piano 6, 50019 Sesto Fiorentino, Firenze, Italy; Institute of Protein Biochemistry, National Research Council, Via P. Castellino, 111, I-80134 Naples, Italy. Laboratory of Microbial and Molecular Evolution, Department of Biology, University of Florence, Via Madonna del Piano 6, 50019 Sesto Fiorentino, Firenze, Italy. Institute of Protein Biochemistry, National Research Council, Via P. Castellino, 111, I-80134 Naples, Italy. Department of Chemical Sciences, University of Naples Federico II, Naples, Italy; School of Biotechnological Sciences, University of Naples Federico II, Complesso Universitario M. S. Angelo, Via Cintia, I-80126 Naples, Italy. Department of Biological and Environmental Sciences (DiSBA-CIBAN), University of Messina, Viale Ferdinando Stagno d'Alcontres 31, I-98166 Messina, Italy. Laboratory of Microbial and Molecular Evolution, Department of Biology, University of Florence, Via Madonna del Piano 6, 50019 Sesto Fiorentino, Firenze, Italy. Electronic address: renato.fani@unifi.it" |
| DOI: | 10.1016/j.micres.2013.09.018 |
| ISSN/ISBN: | 1618-0623 (Electronic) 0944-5013 (Linking) |
| Abstract: | "In this work we analyzed the ability of three Arthrobacter strains (namely TB23, TB26 and CAL618), which were isolated from the Antarctic sponges Haliclonissa verrucosa and Lyssodendrix nobilis, to specifically inhibit the growth of a panel of 40 Burkholderia cepacia complex strains, representing a major cause of infections in patients that are affected by Cystic Fibrosis. The inhibitory activity was due to the synthesis of antimicrobial compounds, very likely volatile organic compounds (VOCs), and was partially dependent on the growth media that were used for Antarctic strains growth. The phylogenetic analysis revealed that two of them (i.e. CAL 618 and TB23) were very close and very likely belonged to the same Arthrobacter species, whereas the strain TB26 was placed in a distant branch. The genome of the strains TB26 and CAL618 was also sequenced and compared with that of the strain TB23. The analysis revealed that TB23 and CAL618 shared more genomic properties (GC content, genome size, number of genes) than with TB26. Since the three strains exhibited very similar inhibition pattern vs Bcc strains, it is quite possible that genes involved in the biosynthesis of antimicrobial compounds very likely belong to the core genome" |
| Keywords: | "Animals Arthrobacter/*genetics/isolation & purification/*metabolism Burkholderia cepacia complex/*drug effects/*growth & development Genome, Bacterial Genomics Phylogeny Porifera/*microbiology Volatile Organic Compounds/metabolism/*pharmacology Arthrobact;" |
| Notes: | "MedlineOrlandini, Valerio Maida, Isabel Fondi, Marco Perrin, Elena Papaleo, Maria Cristiana Bosi, Emanuele de Pascale, Donatella Tutino, Maria Luisa Michaud, Luigi Lo Giudice, Angelina Fani, Renato eng Research Support, Non-U.S. Gov't Germany 2013/11/16 Microbiol Res. 2014 Jul-Aug; 169(7-8):593-601. doi: 10.1016/j.micres.2013.09.018. Epub 2013 Oct 20" |
