| Title: | Functional characterization of olfactory receptors in three Dacini fruit flies (Diptera: Tephritidae) that respond to 1-nonanol analogs as components in the rectal glands |
| Author(s): | Ono H; Miyazaki H; Mitsuno H; Ozaki K; Kanzaki R; Nishida R; |
| Address: | "Graduate School of Agriculture, Kyoto University, Kyoto 606-8502, Japan. Electronic address: onoono@kais.kyoto-u.ac.jp. Graduate School of Agriculture, Kyoto University, Kyoto 606-8502, Japan. Research Center for Advanced Science and Technology, University of Tokyo, Tokyo 153-8904, Japan. JT Biohistory Research Hall, Takatsuki, Osaka 569-1125, Japan" |
| Journal Title: | Comp Biochem Physiol B Biochem Mol Biol |
| DOI: | 10.1016/j.cbpb.2019.110346 |
| ISSN/ISBN: | 1879-1107 (Electronic) 1096-4959 (Linking) |
| Abstract: | "Dacini fruit flies (Tephritidae: Diptera), including destructive pest species, are strongly affected in their reproductive behaviors by semiochemicals. Notably, male lures have been developed for pest management e.g., aromatic compounds for the Oriental fruit fly Bactrocera dorsalis and the melon fruit fly Zeugodacus cucurbitae; terpenic alpha-ionone analogs for the solanaceous fruit fly, B. latifrons. Other than those specific male attractants, 1-nonanol analogs have been noticed as major aliphatic components in the male rectal gland, which is considered as a secretory organ of male sex pheromones. Although multiple semiochemicals associated with the life cycle of Dacini fruit flies have been identified, their behavioral role(s) and chemosensory mechanisms by which the perception occurs have not been fully elucidated. In this study, we conducted RNA sequencing analysis of the chemosensory organs of B. latifrons and Z. cucurbitae to identify the genes coding for chemosensory receptors. Because the skeletons of male attractants are different among Dacini fruit fly species, we analyzed phylogenetic relationships of candidate olfactory receptors (ORs) among the three species. We found that the OR phylogeny reflects the taxonomic relationships of the three species. We further characterized functional properties of OR74a in the three Dacini species to the 1-nonanol analogs related to components in the rectal glands. The three OR74a homologs responded to 1-nonanol, but their sensitivities differed from each other. The OR74a homologs identified from B. dorsalis and Z. cucurbitae responded significantly to 6-oxo-1-nonanol, but not to 1,3-nonanediol and nonyl acetate, indicating similar binding properties of the homologous ORs" |
| Keywords: | "Animals Fatty Alcohols/*pharmacology Insect Proteins/*metabolism Receptors, Odorant/genetics/*metabolism Salt Gland/*metabolism Species Specificity Tephritidae/genetics/*metabolism 1-Nonanol analogs Chemosensory receptor Functional analysis Tephritidae Xe;" |
| Notes: | "MedlineOno, Hajime Miyazaki, Hitomi Mitsuno, Hidefumi Ozaki, Katsuhisa Kanzaki, Ryohei Nishida, Ritsuo eng England 2019/09/10 Comp Biochem Physiol B Biochem Mol Biol. 2020 Jan; 239:110346. doi: 10.1016/j.cbpb.2019.110346. Epub 2019 Sep 6" |
