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Biochem Biophys Res Commun

Title:		Novel Leu-Lys-specific peptidase (Leulysin) produced by gel-entrapped yeast cells
Author(s):		Okada T; Sonomoto K; Tanaka A;
Address:
Journal Title:		Biochem Biophys Res Commun
Year:		1987
Volume:		145
Issue:		1
Page Number:		316 - 322
DOI:		10.1016/0006-291x(87)91323-4
ISSN/ISBN:		0006-291X (Print) 0006-291X (Linking)
Abstract:		"Saccharomyces cerevisiae cells entrapped with a neutral hydrophilic photo-crosslinkable resin prepolymers specifically excreted into a cultured medium a new type of a peptidase, which cleaved Leu-Lys bond of alpha-mating factor. The enzyme was purified by membrane filtration followed by ion exchange chromatography and gel filtration chromatography. The purified enzyme showed a strict substrate specificity on internal Leu-Lys bond. Leu-Lys bond near terminus of a molecule and Leu-X and X-Lys bonds examined so far were not hydrolyzed by the enzyme"
Keywords:		Amino Acid Sequence Endopeptidases/isolation & purification/*metabolism Leucine Lysine Mating Factor Molecular Weight Peptides/metabolism Polyethylene Glycols Saccharomyces cerevisiae/*enzymology Substrate Specificity;
Notes:		"MedlineOkada, T Sonomoto, K Tanaka, A eng 1987/05/29 Biochem Biophys Res Commun. 1987 May 29; 145(1):316-22. doi: 10.1016/0006-291x(87)91323-4"