| Title: | The major cockroach allergen Bla g 4 binds tyramine and octopamine |
| Author(s): | Offermann LR; Chan SL; Osinski T; Tan YW; Chew FT; Sivaraman J; Mok YK; Minor W; Chruszcz M; |
| Address: | "Department of Chemistry and Biochemistry, University of South Carolina, Columbia, SC 29208, USA. Department of Biological Sciences, National University of Singapore, Singapore. Department of Molecular Physiology and Biological Physics, University of Virginia, Charlottesville, VA 22908, USA. Department of Biological Sciences, National University of Singapore, Singapore. Electronic address: dbsmokh@nus.edu.sg. Department of Molecular Physiology and Biological Physics, University of Virginia, Charlottesville, VA 22908, USA. Electronic address: wladek@iwonka.med.virginia.edu. Department of Chemistry and Biochemistry, University of South Carolina, Columbia, SC 29208, USA. Electronic address: chruszcz@mailbox.sc.edu" |
| DOI: | 10.1016/j.molimm.2014.03.016 |
| ISSN/ISBN: | 1872-9142 (Electronic) 0161-5890 (Print) 0161-5890 (Linking) |
| Abstract: | "Bla g 4 is a male cockroach specific protein and is one of the major allergens produced by Blattella germanica (German cockroach). This protein belongs to the lipocalin family that comprises a set of proteins that characteristically bind small hydrophobic molecules and play a role in a number of processes such as: retinoid and pheromone transport, prostaglandin synthesis and mammalian immune response. Using NMR and isothermal titration calorimetry we demonstrated that Bla g 4 binds tyramine and octopamine in solution. In addition, crystal structure analysis of the complex revealed details of tyramine binding. As tyramine and octopamine play important roles in invertebrates, and are counterparts to vertebrate adrenergic transmitters, we speculate that these molecules are physiological ligands for Bla g 4. The nature of binding these ligands to Bla g 4 sheds light on the possible biological function of the protein. In addition, we performed a large-scale analysis of Bla g 4 and Per a 4 (an allergen from American cockroach) homologs to get insights into the function of these proteins. This analysis together with a structural comparison of Blag 4 and Per a 4 suggests that these proteins may play different roles and most likely bind different ligands. Accession numbers: The atomic coordinates and the structure factors have been deposited to the Protein Data Band under accession codes: 4N7C for native Bla g 4 and 4N7D for the Se-Met Bla g 4 structure" |
| Keywords: | "Allergens/chemistry/*immunology/ultrastructure Amino Acid Sequence Animals Cockroaches/*immunology Crystallization Crystallography, X-Ray Hypersensitivity/immunology Insect Proteins/chemistry/*immunology/ultrastructure Male Models, Molecular Octopamine/*i;" |
| Notes: | "MedlineOffermann, Lesa R Chan, Siew Leong Osinski, Tomasz Tan, Yih Wan Chew, Fook Tim Sivaraman, J Mok, Yu-Keung Minor, Wladek Chruszcz, Maksymilian eng P41 GM111244/GM/NIGMS NIH HHS/ R01 GM053163/GM/NIGMS NIH HHS/ GM053163/GM/NIGMS NIH HHS/ Research Support, N.I.H., Extramural England 2014/04/29 Mol Immunol. 2014 Jul; 60(1):86-94. doi: 10.1016/j.molimm.2014.03.016. Epub 2014 Apr 24" |
