| Title: | Evaluation of needle trap micro-extraction and solid-phase micro-extraction: Obtaining comprehensive information on volatile emissions from in vitro cultures |
| Author(s): | Oertel P; Bergmann A; Fischer S; Trefz P; Kuntzel A; Reinhold P; Kohler H; Schubert JK; Miekisch W; |
| Address: | "Department of Anesthesiology and Intensive Care Medicine, Rostock Medical Breath Research Analytics and Technologies, University Medical Center Rostock, Rostock, Germany. Institute of Molecular Pathogenesis at the 'Friedrich-Loeffler-Institut', Jena, Germany" |
| ISSN/ISBN: | 1099-0801 (Electronic) 0269-3879 (Linking) |
| Abstract: | "Volatile organic compounds (VOCs) emitted from in vitro cultures may reveal information on species and metabolism. Owing to low nmol L(-1) concentration ranges, pre-concentration techniques are required for gas chromatography-mass spectrometry (GC-MS) based analyses. This study was intended to compare the efficiency of established micro-extraction techniques - solid-phase micro-extraction (SPME) and needle-trap micro-extraction (NTME) - for the analysis of complex VOC patterns. For SPME, a 75 mum Carboxen(R)/polydimethylsiloxane fiber was used. The NTME needle was packed with divinylbenzene, Carbopack X and Carboxen 1000. The headspace was sampled bi-directionally. Seventy-two VOCs were calibrated by reference standard mixtures in the range of 0.041-62.24 nmol L(-1) by means of GC-MS. Both pre-concentration methods were applied to profile VOCs from cultures of Mycobacterium avium ssp. paratuberculosis. Limits of detection ranged from 0.004 to 3.93 nmol L(-1) (median = 0.030 nmol L(-1) ) for NTME and from 0.001 to 5.684 nmol L(-1) (median = 0.043 nmol L(-1) ) for SPME. NTME showed advantages in assessing polar compounds such as alcohols. SPME showed advantages in reproducibility but disadvantages in sensitivity for N-containing compounds. Micro-extraction techniques such as SPME and NTME are well suited for trace VOC profiling over cultures if the limitations of each technique is taken into account" |
| Keywords: | "Aldehydes/analysis Bacteriological Techniques/*methods Cells, Cultured Gas Chromatography-Mass Spectrometry Ketones/analysis Limit of Detection Linear Models Mycobacterium avium/cytology/metabolism Nitrogen Compounds/analysis Reproducibility of Results So;" |
| Notes: | "MedlineOertel, Peter Bergmann, Andreas Fischer, Sina Trefz, Phillip Kuntzel, Anne Reinhold, Petra Kohler, Heike Schubert, Jochen K Miekisch, Wolfram eng RE 1098/4-1RE 1098/4-2SCHU 1960/-2SCHU 1960/4-1/Deutsche Forschungsgemeinschaft/ England 2018/05/16 Biomed Chromatogr. 2018 Oct; 32(10):e4285. doi: 10.1002/bmc.4285. Epub 2018 Jun 5" |
