« Previous AbstractExhaled volatile substances mirror clinical conditions in pediatric chronic kidney disease    Next AbstractCryptic termites avoid predatory ants by eavesdropping on vibrational cues from their footsteps »

Nutrients

Title:		"The Effects of Prebiotic Supplementation with OMNi-LOGiC((R)) FIBRE on Fecal Microbiome, Fecal Volatile Organic Compounds, and Gut Permeability in Murine Neuroblastoma-Induced Tumor-Associated Cachexia"
Author(s):		Obermuller B; Singer G; Kienesberger B; Klymiuk I; Sperl D; Stadlbauer V; Horvath A; Miekisch W; Gierschner P; Grabherr R; Gruber HJ; Semeraro MD; Till H; Castellani C;
Address:		"Department of Paediatric and Adolescent Surgery, Medical University of Graz, 8036 Graz, Austria. Department of Biomedical Research, Medical University of Graz, 8036 Graz, Austria. Core Facility Molecular Biology, Center for Medical Research, Medical University of Graz, 8036 Graz, Austria. Division of Paediatric Haematology and Oncology, Department of Pediatrics and Adolescent Medicine, Medical University of Graz, 8036 Graz, Austria. Division of Gastroenterology and Hepatology, Department of Internal Medicine, Medical University of Graz, 8036 Graz, Austria. Center for Biomarker Research in Medicine (CBmed), 8036 Graz, Austria. Rostock Medical Breath Research Analytics and Technologies (ROMBAT), Department of Anesthesiology and Intensive Care, Rostock University Medical Center, 18057 Rostock, Germany. Department of Biotechnology, University of Natural Resources and Life Sciences, 1190 Vienna, Austria. Clinical Institute of Medical and Chemical Laboratory Diagnostics, Medical University of Graz, 8036 Graz, Austria"
Journal Title:		Nutrients
Year:		2020
Volume:		20200708
Issue:		7
Page Number:		-
DOI:		10.3390/nu12072029
ISSN/ISBN:		2072-6643 (Electronic) 2072-6643 (Linking)
Abstract:		"Malignant diseases can cause tumor-associated cachexia (TAC). Supplementation with prebiotic non-digestible carbohydrates exerts positive metabolic effects in experimental oncologic diseases. The aim of this project was to assess the effect of prebiotic supplementation with OMNi-LOGiC((R)) FIBRE on intestinal microbiome, bacterial metabolism, gut permeability, and inflammation in a murine model of neuroblastoma (NB)-associated TAC. For this study, 2,000,000 NB cells (MHH-NB11) were implanted into athymic mice followed by daily supplementation with water or 200 mg prebiotic oligosaccharide (POS) OMNi-LOGiC((R)) FIBRE (NB-Aqua, n = 12; NB-POS, n = 12). Three animals of each tumor group did not develop NB. The median time of tumor growth (first visibility to euthanasia) was 37 days (IQR 12.5 days) in the NB-Aqua group and 37 days (IQR 36.5 days) in the NB-POS group (p = 0.791). At euthanasia, fecal microbiome and volatile organic compounds (VOCs), gut permeability (fluorescein isothiocyanate-dextran (FITC-dextran), and gut barrier markers were measured. Values were compared to sham animals following injection of culture medium and gavage of either water or OMNi-LOGiC((R)) FIBRE (SH-Aqua, n = 10; SH-POS, n = 10). Alpha diversity did not differ significantly between the groups. Principal coordinate analysis (PCoA) revealed clustering differences between Aqua and POS animals. Both NB and POS supplementation led to taxonomic alterations of the fecal microbiome. Of 49 VOCs, 22 showed significant differences between the groups. NB animals had significantly higher gut permeability than Aqua animals; POS did not ameliorate these changes. The pore and leak pathways of tight junctions did not differ between groups. In conclusion, our results suggest that NB-induced TAC causes increased gut permeability coupled with compositional changes in the fecal microbiome and VOC profile. Prebiotic supplementation with OMNi-LOGiC((R)) FIBRE seemed to induce modifications of the fecal microbiome and VOC profile but did not improve gut permeability"
Keywords:		"Animals Cachexia/etiology/*metabolism/*microbiology Dietary Carbohydrates/administration & dosage/pharmacology Dietary Fiber/administration & dosage/pharmacology *Dietary Supplements Disease Models, Animal Feces/*chemistry Gastrointestinal Microbiome/*dru;"
Notes:		"MedlineObermuller, Beate Singer, Georg Kienesberger, Bernhard Klymiuk, Ingeborg Sperl, Daniela Stadlbauer, Vanessa Horvath, Angela Miekisch, Wolfram Gierschner, Peter Grabherr, Reingard Gruber, Hans-Jurgen Semeraro, Maria D Till, Holger Castellani, Christoph eng 867991/Osterreichische Forschungsforderungsgesellschaft/ Switzerland 2020/07/12 Nutrients. 2020 Jul 8; 12(7):2029. doi: 10.3390/nu12072029"