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Environ Toxicol Pharmacol

Title:		Induction of in vitro EROD activity and in vivo caffeine metabolism in two species of New Zealand birds
Author(s):		Numata M; Fawcett JP; Rosengren RJ;
Address:		"School of Pharmacy, University of Otago, PO Box 913, Dunedin, New Zealand"
Journal Title:		Environ Toxicol Pharmacol
Year:		2008
Volume:		20071203
Issue:		3
Page Number:		358 - 364
DOI:		10.1016/j.etap.2007.11.005
ISSN/ISBN:		1382-6689 (Print) 1382-6689 (Linking)
Abstract:		"In birds, induction of cytochrome P4501A (CYP1A) is usually assessed as liver microsomal ethoxyresorufin O-deethylase (EROD) activity, but in mammals, it can be determined by a caffeine metabolism blood test. We investigated both of these measures in two species of New Zealand birds. Administration of a model CYP1A inducer, beta-naphthoflavone (BNF) (80mg/kg i.p. twice 2 days apart), to paradise shelducks (Tadorna variegata; herbivore) and southern black-backed gulls (Larus dominicanus; omnivore) (n=5 or 6) caused marked increases in EROD activity (80- and 20-fold, respectively). In both species, BNF treatment also caused significant increases (>8-fold) in caffeine metabolism determined prior to sacrifice as the serum concentration ratio of the major metabolite, paraxanthine, to caffeine, after caffeine administration (1mg/kg i.p.). The results suggest in vivo caffeine metabolism is a potentially useful non-destructive biomarker of CYP1A induction in wild birds"
Keywords:
Notes:		"PubMed-not-MEDLINENumata, Mihoko Fawcett, J Paul Rosengren, Rhonda J eng Netherlands 2008/05/01 Environ Toxicol Pharmacol. 2008 May; 25(3):358-64. doi: 10.1016/j.etap.2007.11.005. Epub 2007 Dec 3"