| Title: | Detailed qualitative analysis of honeybush tea (Cyclopia spp.) volatiles by comprehensive two-dimensional gas chromatography coupled to time-of-flight mass spectrometry and relation with sensory data |
| Author(s): | Ntlhokwe G; Muller M; Joubert E; Tredoux AGJ; de Villiers A; |
| Address: | "Department of Chemistry and Polymer Science, Stellenbosch University, Private Bag X1, Matieland, 7602, South Africa. Department of Food Science, Stellenbosch University, Private Bag X1, Matieland, 7602, South Africa. Department of Food Science, Stellenbosch University, Private Bag X1, Matieland, 7602, South Africa; Post-Harvest and Wine Technology Division, Agricultural Research Council (ARC), Infruitec-Nietvoorbij, Private Bag X5026, Stellenbosch, 7566, South Africa. Department of Chemistry and Polymer Science, Stellenbosch University, Private Bag X1, Matieland, 7602, South Africa. Electronic address: atredoux@sun.ac.za. Department of Chemistry and Polymer Science, Stellenbosch University, Private Bag X1, Matieland, 7602, South Africa. Electronic address: ajdevill@sun.ac.za" |
| DOI: | 10.1016/j.chroma.2017.08.054 |
| ISSN/ISBN: | 1873-3778 (Electronic) 0021-9673 (Linking) |
| Abstract: | "The volatile composition of honeybush (Cyclopia) species was studied by comprehensive two-dimensional gas chromatography coupled to time-of-flight mass spectrometry (GCxGC-TOF-MS). Headspace-solid phase micro-extraction (HS-SPME) was used to extract the volatile compounds from tea infusions prepared from the three species C. genistoides, C. maculata and C. subternata. A total of 287 compounds were identified, 101 of which were confirmed using reference standards, while the remainder were tentatively identified using mass spectral and retention index (RI) data. The identification power of TOF-MS enabled the tentative identification of 147 compounds for the first time in honeybush tea. The majority of the compounds identified were common to all three Cyclopia species, although there were differences in their relative abundances, and some compounds were unique to each of the species. In C. genistoides, C. maculata and C. subternata 265, 257 and 238 compounds were identified, respectively. Noteworthy was the tentative identification of cinnamaldehyde in particular C. maculata samples, which points to the likely contribution of this compound to their distinct sensory profiles. This study emphasises the complexity of honeybush tea volatile composition and confirms the power of GCxGC combined with TOF-MS for the analysis of such complex samples" |
| Keywords: | Food Analysis/*methods *Gas Chromatography-Mass Spectrometry Holoprosencephaly/*complications Solid Phase Microextraction Tea/*chemistry Volatile Organic Compounds/*chemistry Comprehensive two-dimensional gas chromatography (GC x GC) Honeybush tea Sensory; |
| Notes: | "MedlineNtlhokwe, Gaalebalwe Muller, Magdalena Joubert, Elizabeth Tredoux, Andreas G J de Villiers, Andre eng Netherlands 2017/09/06 J Chromatogr A. 2018 Feb 9; 1536:137-150. doi: 10.1016/j.chroma.2017.08.054. Epub 2017 Aug 24" |
