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Biochem Biophys Res Commun

Title:		Distribution of RGS4 mRNA in mouse brain shown by in situ hybridization
Author(s):		Nomoto S; Adachi K; Yang LX; Hirata Y; Muraguchi S; Kiuchi K;
Address:		"Laboratory for Genes of Motor Systems, RIKEN, Nagoya, Japan"
Journal Title:		Biochem Biophys Res Commun
Year:		1997
Volume:		241
Issue:		2
Page Number:		281 - 287
DOI:		10.1006/bbrc.1997.7802
ISSN/ISBN:		0006-291X (Print) 0006-291X (Linking)
Abstract:		"RGS proteins (regulators of G protein signaling) are a newly identified group of proteins that negatively regulate G proteins. Some, including RGS4, have been shown to act as GTPase-activating proteins for several members of the Gi alpha subunits and the Gq alpha subunit. However, the signaling pathways of G protein-coupled receptors regulated by RGS4 are not well understood. We isolated the mouse RGS4 cDNA to investigate the roles of RGS4 protein in mouse brain. Expression of mouse RGS4 in yeast stimulated adaptation to mating pheromone, suggesting that it negatively regulates that G protein-mediated signaling pathway. Northern blot analysis of various mouse tissues revealed that RGS4 is expressed at high levels in brain, moderately low levels in heart, and very low levels in lung, liver, and skeletal muscle. In situ hybridization of mouse brain showed RGS4 mRNA mainly in the cerebral cortex, hippocampus, anterior olfactory nucleus, piriform cortex, olfactory tubercle, caudate-putamen, nucleus accumbens, islands of Calleja, substantia nigra, amygdala, the granular layer of cerebellum, middle cerebellar peduncle, and perifacial zone. These results suggest that RGS4 is expressed in various types of cells in the brain and may regulate multiple G protein-mediated signaling pathways"
Keywords:		"Animals *Brain Chemistry Calcium-Calmodulin-Dependent Protein Kinases/antagonists & inhibitors Cloning, Molecular GTP-Binding Proteins/metabolism In Situ Hybridization Male Mating Factor Mice Mice, Inbred C57BL Molecular Sequence Data Nerve Tissue Protein;"
Notes:		"MedlineNomoto, S Adachi, K Yang, L X Hirata, Y Muraguchi, S Kiuchi, K eng Comparative Study 1998/01/13 Biochem Biophys Res Commun. 1997 Dec 18; 241(2):281-7. doi: 10.1006/bbrc.1997.7802"