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Phytomedicine

Title:		"An integration of UPLC-DAD/ESI-Q-TOF MS, GC-MS, and PCA analysis for quality evaluation and identification of cultivars of Chrysanthemi Flos (Juhua)"
Author(s):		Nie J; Xiao L; Zheng L; Du Z; Liu D; Zhou J; Xiang J; Hou J; Wang X; Fang J;
Address:		"Hubei Institute for Food and Drug Control, Wuhan 430064, China. School of Pharmacy, Hubei Key Laboratory of Natural Medicinal Chemistry and Resource Evaluation, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China. School of Pharmacy, Hubei Key Laboratory of Natural Medicinal Chemistry and Resource Evaluation, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China. Electronic address: fangjb@hust.edu.cn"
Journal Title:		Phytomedicine
Year:		2019
Volume:		20181221
Issue:
Page Number:		152803 -
DOI:		10.1016/j.phymed.2018.12.026
ISSN/ISBN:		1618-095X (Electronic) 0944-7113 (Linking)
Abstract:		"BACKGROUND: Chrysanthemi Flos (CF), as a popular traditional Chinese medicine (TCM), has five main cultivars in China, namely 'Chuju', 'Boju', 'Gongju', 'Huaiju' and 'Hangju'. Due to their habitats and processing methods, great quality variations occur yet no systematical study has ever been carried out to evaluate such variations. PURPOSE: In this study, we aim to establish a new approach that can serve both as a quality control method and as an identification method for cultivars of CF. METHOD: The components in CF samples were identified by a combination of UPLC-ESI-Q-TOF/MS and GC/MS. Furthermore, a multimodal quantitative method was established by UPLC-UV coupled with principal component analysis (PCA) and the similarity evaluation system (SES), which was used to control and identify four cultivars of CF. RESULTS: 18 compounds of flavonoids and caffeoylquinic acids were identified and ten of them were quantified using UPLC-ESI-Q-TOF/MS. Different cultivars of CF could be clearly distinguished with the fingerprints evaluation and principal component analysis (PCA). A total of 74 volatile compounds were detected by GC/MS. The distinctness of volatile components was observed. By the combination of UPLC-ESI-Q-TOF/MS and GC/MS, an identification and quality control method for CF was successfully established. CONCLUSION: The combination of UPLC-ESI-Q-TOF/MS and GC/MS could act as a comprehensive multimodal method for both identification and quality control of herbal medicines. This study provided new insights into the overall evaluation method for herbal medicines possessing different cultivars"
Keywords:		"China Chromatography, High Pressure Liquid/methods Chrysanthemum/*chemistry Drugs, Chinese Herbal/*analysis/chemistry Flavonoids/analysis Flowers/chemistry Gas Chromatography-Mass Spectrometry/*methods/statistics & numerical data Principal Component Analy;"
Notes:		"MedlineNie, Jing Xiao, Ling Zheng, Lamei Du, Zhifeng Liu, Dan Zhou, Jiewen Xiang, Jin Hou, Junjie Wang, Xiaogang Fang, Jinbo eng Germany 2019/04/22 Phytomedicine. 2019 Jun; 59:152803. doi: 10.1016/j.phymed.2018.12.026. Epub 2018 Dec 21"